フラットBARタンパク質は,クラトリンで覆われた穴の底部でのアクチンポリメリゼーションを促進する
Leonardo Almeida-Souza1, Rene A W Frank1, Javier García-Nafría1
1MRC Laboratory of Molecular Biology, Francis Crick Avenue, Cambridge CB2 0QH, UK.
Cell
|June 12, 2018
まとめ
FCHSD2タンパク質は,哺乳類のクラトリン媒介性内分細胞症 (CME) に不可欠である. アクチンポリメリゼーションを活性化し,プラズマ膜のアクチン細胞骨格を組織することによって,膀の形成とリガンドの吸収を促進します.
科学分野:
- 細胞生物学
- 分子生物学と細胞生物学
- 生物化学
背景:
- クラトリン媒介性内細胞症 (CME) は細胞のプロセスに不可欠である.
- アクチン細胞骨格の組織は,哺乳類のCMEにとって重要ですが,完全に理解されていません.
研究 の 目的:
- 哺乳類のCMEにおけるFCHSD2の役割を調査する.
- エンドサイトーシス中のアクチンポリメリゼーションにおけるFCHSD2の分子メカニズムを解明する.
主な方法:
- FCHSD2の機能を評価する研究
- タンパク質の相互作用と内細胞穴への誘導の分析
- FCHSD2によるアクチン活性化のメカニズムを調査する.
主要な成果:
- FCHSD2の削除はリガンドの吸収を阻害し,ピット成熟を遅らせます.
- FCHSD2は,インターセクチンとのSH3-SH3相互作用により,内細胞穴に誘導される.
- FCHSD2のF-BARドメインはプラズマ膜に結合し,N-WASP募集を通じてアクチンポリメリゼーションを活性化します.
結論:
- FCHSD2は哺乳類のCMEにおけるアクチンポリメリゼーションの主要な活性化剤である.
- FCHSD2はプラズマ膜をアクチン細胞骨格に結合し,内細胞の成熟を促す.
- この研究は,エンドサイトーシスのアクチン調節のための新しいメカニズムを明らかにしています.
関連する概念動画
Clathrin Coated Vesicles
9.4K
Clathrin-coated vesicles use endocytosis to transport receptors and lysosomal hydrolases from the Golgi to the lysosome in the late secretory pathway. Clathrin-mediated endocytosis was the first described endocytic process, and Clathrin-coated vesicles remain one of the most well-studied transport vesicles. The molecular machinery that generates clathrin-coated vesicles comprises over 50 proteins that precisely coordinate vesicle formation. Cell surface receptors concentrated in indented sites...
9.4K
Actin Polymerization
8.6K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶ nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
8.6K
Actin Polymerization and Cell Motility
6.7K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
6.7K
Bar Graph
23.0K
A bar graph is also called a bar chart and consists of bars that are separated from each other. It either uses horizontal or vertical bars to show comparisons among categories. The bars can be rectangles, or they can be rectangular boxes (used in three-dimensional plots). One axis of the graph represents the specific categories being compared, and the other axis shows a discrete value. In this graph, the length of the bar for each category is proportional to the number or percent of individuals...
23.0K
Multiple Bar Graph
10.1K
As the name suggests, a multiple bar graph is the same as a bar graph but has multiple bars to depict relationships between different data values. One can include as many parameters as possible. However, each parameter must have the same unit of measurement.
Each bar or column in the multiple bar graph represents a data value. These graphs are used primarily in interrelating two or more sets of data. The categories of different kinds of data are listed along the horizontal or x-axis, whereas...
Each bar or column in the multiple bar graph represents a data value. These graphs are used primarily in interrelating two or more sets of data. The categories of different kinds of data are listed along the horizontal or x-axis, whereas...
10.1K
The Eukaryotic Promoter Region
18.9K
The eukaryotic promoter region is a segment of DNA located upstream of a gene. It contains an RNA polymerase binding site, a transcription start site, and several cis-regulatory sequences. The proximal promoter region is located in the vicinity of the gene and has cis-regulatory sequences and the core promoter. The core promoter is the binding site for RNA polymerase and is usually located between -35 and +35 nucleotides from the transcription start site. The distal promoter regions are...
18.9K


