マグネティック・レビテーションを用いた高通量密度測定
Shencheng Ge1, Yunzhe Wang1, Nicolas J Deshler1
1Department of Chemistry & Chemical Biology , Harvard University , 12 Oxford Street , Cambridge , Massachusetts 02138 , United States.
Journal of the American Chemical Society
|June 12, 2018
まとめ
この研究は,細胞を含む様々な材料の密度測定のための高通量磁気浮揚 (MagLev) システムを提示します. 統合システムは96穴のプレートとスキャナーを使用して,効率的でラベルのない分析を行います.
科学分野:
- 分析化学
- 生物化学
- 材料科学
背景:
- マグネティック・レビテーション (MagLev) は,密度ベースの分析を提供しますが,高通量機能はありません.
- 現在のマグレフ・メソッドは複数のサンプルを同時に分析するのに最適化されていません.
- 密度に基づく分類は様々な科学分野において極めて重要です.
研究 の 目的:
- 密度測定のための統合された高通量分析システムを開発する.
- 標準の96wellプレートとの互換性のためにMagLevテクノロジーを適応させる.
- 生物細胞を含む様々な二磁性物質の密度ベースの分析を可能にします.
主な方法:
- 磁場を再設計して 96穴のプレートで マグレフを起動させる
- フラットベッドスキャナーとサンプルイメージングのための光学部品を組み込みました.
- 磁気浮揚を活用して,様々な磁気質のサンプルを密度測定した.
主要な成果:
- 統合されたマグレフシステムを用いて高通量密度測定を成功させた.
- 生物学的 (ヒトの赤血球) と非生物学的サンプル (液体,固体,粉末,ビーズ) の両方でシステムの互換性を検証した.
- 磁気浮揚によるラベルフリー密度分析を達成した.
結論:
- 開発された統合MagLevシステムは,以前のスループット制限を克服します.
- この技術は,材料の分析,分離,生化学研究における新しいアプリケーションを可能にします.
- このシステムは,物質の普遍的な性質を用いた密度の特徴付けのためのシンプルな,ラベルフリーな方法を提供します.
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