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著者訂正:CRISPR活性化による刺激反応性免疫強化剤の発見
Dimitre R Simeonov1,2,3,4, Benjamin G Gowen4,5, Mandy Boontanrart4,5
1Biomedical Sciences Graduate Program, University of California, San Francisco, California, 94143, USA.
Nature
|June 15, 2018
まとめ
再分析されたデータは,実験的変異を補正した後,Il2ra増強剤消去 (EDEL) のマウスにおける調節性T細胞 (Treg) パーセントの微妙な減少を示した. この発見は,EDELマウスモデルにおけるTreg細胞の動態を明確にします.
科学分野:
- 免疫学
- 遺伝学
- 分子生物学
背景:
- 調節性T細胞は免疫ホメオスタシスの維持に不可欠です.
- Il2ra増強剤はTreg細胞機能に作用する.
- Il2ra増強剤消去 (EDEL) のマウスの以前の分析では,Treg細胞の割合で有意な差異は示されなかった.
研究 の 目的:
- Il2ra増強剤の消去 (EDEL) がTreg細胞の割合に与える影響を再評価する.
- ネズミの研究における遺伝子型割り当ての誤りとコホート間の変動を修正する.
主な方法:
- EDELと野生型 (WT) のマウスにおける安定状態のTreg細胞割合の分析.
- 誤ったマウスの遺伝子型を修正する.
- 実験コホート間の差異を考慮するデータの正常化.
主要な成果:
- EDELマウスの初期分析では,Treg細胞の割合に違いはなかった.
- ゲノタイプ修正とコホート変異の正常化の後,EDEL Treg細胞の割合が微妙に減少した.
- この発見により,以前に公表された数字と記述を修正する必要がありました.
結論:
- Il2ra増強剤消去 (EDEL) 菌株は,データが適切に正常化されると,Treg細胞の割合が微妙に低下します.
- 特定された誤りは,原書に記載された主な結論や他の重要なデータに影響を与えない.
- Tレグ細胞の調節におけるIl2ra増強剤の正確な役割については,さらなる調査が必要である.
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