SAMDI質量スペクトロメトリーによる高通量実験のための分析物の不動化
Kazi Y Helal, Azmain Alamgir, Eric J Berns
1Department of Cell and Molecular Biology , Northwestern University Feinberg School of Medicine , Chicago , Illinois 60611 , United States.
Journal of the American Chemical Society
|June 15, 2018
まとめ
この研究は,質量スペクトロメトリー分析のための表面に分子を固定するための痕跡のない化学方法を導入します. このテクニックは分子活動の変化を避け,薬剤発見と反応最適化のための高通量スクリーニングを可能にします.
科学分野:
- 分析化学
- 生物化学
- 有機化学
背景:
- ラベルなしの質量スペクトロメトリアッセイは,高通量反応分析を容易にする.
- 現在の方法は,反応物/産物不動化のための機能群をしばしば必要とし,潜在的に分子活動を変化させる.
- 表面ベースの測定では,痕跡のない固定化技術が必要である.
研究 の 目的:
- 多様な分子を自己組み立てモノレイヤー (SAM) に共振的に結合させるための無痕方法を開発する.
- 表面ベースの質量スペクトロメトリー分析を可能にし,興味のある分子の固有の性質を変更しないようにします.
- 酵素の特徴と化学反応のモニタリングにおけるこの方法の有用性を実証する.
主な方法:
- 3トリフローロメチル3フェニルディアジリン群によるSAMの機能化.
- 紫外線照射によるカルベンの中間物質の光分解生成
- カーベンを様々な分子結合に共振的に挿入して固定する.
- 表面補助レーザー消水/イオン化 (SAMDI) 質量スペクトロメトリーを使用して固定されたアダクトの分析.
主要な成果:
- 機能化されたSAMに 微粒子を成功裏に固定した
- SAMDI質量スペクトロメトリーによる固定されたアダクトの検出.
- P450薬代謝酵素を特徴付ける方法の適用
- 開発した技術を用いてスズキ-ミヤウラ結合反応の監視.
結論:
- 開発された痕跡のない固定化方法は,表面ベースの質量スペクトロメトリに有効です.
- このアプローチは生化学と化学の研究に不可欠な分子の本来の活動を保存します.
- この方法は,様々な科学分野における高通量スクリーニング,反応発見および最適化に重要な可能性を秘めています.
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