老化 し て いる ドロソフィラ の 脳 の 単細胞 トランスクリプトーム アトラス
Kristofer Davie1, Jasper Janssens1, Duygu Koldere1
1VIB Center for Brain & Disease Research, KU Leuven, Leuven 3000, Belgium; Department of Human Genetics KU Leuven, Leuven 3000, Belgium.
Cell
|June 19, 2018
まとめ
この研究は 成虫の脳内の 全ての細胞タイプを 生涯にわたってマッピングします 果物ハエの老化した脳は RNAの含有量が減少していますが ニューロンのアイデンティティは保たれています
科学分野:
- 神経科学
- ゲノミクス
- 老化に関する研究
背景:
- 脳の細胞の多様性と 老化中の変化を理解することは 極めて重要です
- 大人のドロソフィラ・メラノガスターの脳細胞の複雑性と制御状態はほとんど特徴づけられていない.
研究 の 目的:
- 成人フルーツハエの脳全体の 単細胞トランスクリプトームアトラスを作成します
- 果物ハエの寿命を通して 細胞の多様性と遺伝子調節がどのように変化するかを調べる
主な方法:
- 成人ドロソフィラ・メラノガスターの脳全体の単細胞RNA配列解析
- 高解像度のセルタイプ識別のためのセルクラスタリングとサブクラスタリング.
- SCENIC (サブセルラー実験ネットワーク推論および相関エンジン) を使用した遺伝子ネットワーク分析.
主要な成果:
- 87の異なる細胞群を特定し 細胞の多様性を明らかにした
- 遺伝子ネットワークの分析により,エネルギー消費に関連する規制の異質性が明らかになった.
- ニューロンのアイデンティティを損なうことなく 歳をとる過程でRNAの指数関数的な減少が観察されました
結論:
- 発達したアトラスは 正常な脳のほぼ全ての細胞の 詳細な地図を提供します
- この発見は 単細胞レベルで 老化過程の洞察を与えてくれます
- 統合されたプラットフォーム (SCope) は,脳細胞の転写状態の比較分析を容易にする.
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