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Updated: May 10, 2026

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy
09:03

Functional Imaging of Viral Transcription Factories Using 3D Fluorescence Microscopy

Published on: January 18, 2018

2つの異なった転写因子は,HSVのチミジンキナーゼプロモーターにインビトロで結合する

K A Jones, K R Yamamoto, R Tjian

    Cell
    |September 1, 1985
    PubMed
    まとめ

    この研究は,ヘルペスウイルスのチミジンキナーゼ (TK) プロモーターのためのインビトロ転写システムを特徴づけています. 遺伝子発現に不可欠な特定のタンパク質結合部位を明らかにし,Sp1およびCCAAT結合因子を含む.

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    Enhanced Yeast One-hybrid Screens To Identify Transcription Factor Binding To Human DNA Sequences

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    Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models13:47

    Lentiviral Vector Platform for the Efficient Delivery of Epigenome-editing Tools into Human Induced Pluripotent Stem Cell-derived Disease Models

    Published on: March 29, 2019

    関連する概念動画

    Transcription Factors02:16

    Transcription Factors

    Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
    RNA Polymerase II Accessory Proteins02:36

    RNA Polymerase II Accessory Proteins

    Proteins that regulate transcription can do so either via direct contact with RNA Polymerase or through indirect interactions facilitated by adaptors, mediators, histone-modifying proteins, and nucleosome remodelers. Direct interactions to activate transcription is seen in bacteria as well as in some eukaryotic genes. In these cases, upstream activation sequences are adjacent to the promoters, and the activator proteins interact directly with the transcriptional machinery. For example, in...
    Co-activators and Co-repressors02:04

    Co-activators and Co-repressors

    Gene transcription is regulated by the synergistic action of several proteins that form a complex at a gene regulatory site. This is observed in eukaryotes, where the regulation of gene expression is a complex process. Regulatory proteins in eukaryotes can broadly be classified into two types – regulators that bind directly to specific DNA sequences and co-regulators that associate with regulatory proteins but cannot directly bind to the DNA. These co-regulators are further divided into...
    Eukaryotic Transcription Activators02:42

    Eukaryotic Transcription Activators

    Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
    The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These domains are...
    Eukaryotic Transcription Inhibitors01:52

    Eukaryotic Transcription Inhibitors

    Certain biochemical processes, such as embryonic development and cell growth regulation, depend on the repression of specific genes. DNA binding proteins known as eukaryotic transcription inhibitors regulate the repression of gene expression in eukaryotes. The presence of these inhibitors at the required location and time in the cell is triggered by the presence of hormones and additional signals from other cells.
    Eukaryotic transcription inhibitors usually contain two distinct domains, a DNA...
    General Transcription Factors01:30

    General Transcription Factors

    Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...