免疫模倣設計細胞は,マウスをMRSA感染から保護する
Ying Liu1, Peng Bai1, Anne-Kathrin Woischnig2
1Department of Biosystems Science and Engineering, ETH Zurich, Mattenstrasse 26, 4058 Basel, Switzerland.
Cell
|June 26, 2018
まとめ
研究 者 たち は,メチシリン 耐性 Staphylococcus aureus (MRSA) を 検出 し,破壊 する ため に 哺乳類 の 細胞 を 設計 し まし た. この新しい合成生物学的アプローチは 抗生物質に対する有望な代替手段として 耐性細菌の感染を治療します
科学分野:
- 合成生物学
- 免疫学
- 微生物学
背景:
- 抗生物質耐性菌感染は,メチシリン耐性黄金球菌 (MRSA) など,世界的な健康上の重大な脅威となっています.
- 現在の治療法は 耐性菌が増えるため 効果が低下しています
- MRSA感染は根絶するのが困難で,重度の侵襲性疾患につながる可能性があります.
研究 の 目的:
- 哺乳類の細胞ベースのシステムを設計・開発し,バクテリア感染を自律的に検出・排除する.
- 細菌の存在を感知し 反応する 閉じたループの遺伝子ネットワークを作ります
- MRSAに対する人工免疫模倣設計細胞の治療可能性を評価する.
主な方法:
- 細菌の感知のためのヒトのトールのような受容体を用いて哺乳類の細胞で合成遺伝子回路を開発した.
- バクテリア分解酵素である リススタフィンを発現させるための回路を設計した
- マウス外体感染モデルに 遺伝子組み換え細胞を埋め込みました
主要な成果:
- エンジニアリングした細胞は 生きたスタフィロコーキの迅速かつ効果的な検出と除去を証明しました
- ミクロカプセルインプラントはマウスのMRSA感染とバイオフィルム形成を91%減少させた.
- 急性MRSA感染症の治癒率は100%で ヴァンコマイシンよりも優れています
結論:
- 免疫模倣設計細胞は,抗生物質耐性感染症と戦うための有効な治療戦略です.
- この合成生物学的アプローチは 病原性細菌感染症の早期発見,予防,治療の可能性を示しています
- このシステムは 抗生物質の時代を 迎える有望な代替手段です
キーワード:
トール型受容体抗生物質に対する耐性バイオフィルム細胞療法カプセル化インプラント関連感染症リソスタフィンメチチリンに耐性がある Staphylococcus aureusネズミの組織ケージ感染モデル合成遺伝子回路さらに関連する動画
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