非天然のフラボプロテインの磁気感受性ラジカル光化学

Tilo M Zollitsch¹, Lauren E Jarocha¹, Chris Bialas²

¹Department of Chemistry , University of Oxford, Physical and Theoretical Chemistry Laboratory , Oxford OX1 3QZ , United Kingdom.

Journal of the American Chemical Society

|June 26, 2018

まとめ

研究者たちは鳥がナビゲーションに使用する分子である暗号クロームを研究するためにタンパク質のモデルを設計しましたこれらのモデルは,暗号クロームを模倣しています.

科学分野:

バイオ物理学
写真化学
タンパク質工学

背景:

地球の磁場は移動する鳥のナビゲーションを光に依存する化学コンパスで影響する.
フラボプロテインである暗号クロームは提案された感覚分子で青い光の誘発による電子移転を利用して根幹のペアを形成します
この生物学的ナビゲーションメカニズムを解読するには暗号クロムの磁気感受性を理解することが重要です

研究の目的:

クリプトクロームの磁気感受性を制御する要因を調査する.
磁場効果に起因する光化学と一時的なラジカルを特徴づける.
クリプトクローム機能の研究における設計されたタンパク質モデルの有用性を評価する.

主な方法:

異なる距離 (0.6,1.1,1.7 nm) でトリプトファンとリボフラビンを組み込んだ4α-ヘリクセルのタンパク質モデルの新規設計.
設計されたフラボプロテインモデルの光化学を分析するために空洞のリングダウンスペクトロスコーピーを利用しました.
磁場相互作用に関与する一時的なラジカルのアイデンティティと運動を決定した.

主要な成果:

設計されたタンパク質模型は,暗号色光化学の重要な側面を成功裏に真似しました.
マケットの磁場効果は自然の暗号クロームと驚くほど似ています
磁気感受性メカニズムに洞察を与える,一時的なラジカルとその動態を特定した.

結論:

構造的な違いにもかかわらず,タンパク質の模型は暗号クロームの磁気感受性を研究するための貴重なモデルとして機能します.
この発見は,鳥の磁気受容の基礎として,ラジカルペアメカニズムを支持する.
このアプローチは,生物学的磁気センサーの光物理的および化学的基礎を解剖するための強力なツールを提供します.

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