生体細胞における感受性RNAイメージングのための遺伝的にコードされた触媒式ヘアピン組
Aruni P K K Karunanayake Mudiyanselage1, Qikun Yu1, Mark A Leon-Duque1
1Department of Chemistry , University of Massachusetts , Amherst , Massachusetts 01003 , United States.
Journal of the American Chemical Society
|June 27, 2018
まとめ
遺伝的にコードされたRNA回路は 生きている細胞の 敏感なRNA画像を可能にします このプログラム可能なシステムは 触媒式ヘアピンアセンブリと 分割されたブロッコリーアプタマーを使用して 検出と細胞分析を強化します
科学分野:
- 分子生物学
- バイオテクノロジー
- ナノテクノロジー
背景:
- DNAとRNAのナノテクノロジーは ダイナミックな分子装置を進歩させています
- 触媒式ヘアピンアセンブリ (CHA) のような酵素のない核酸回路は,バイオアナリシスにとって有望である.
- 細胞内伝達と分解の課題は合成核酸回路を制限する.
研究 の 目的:
- 敏感な細胞内RNAイメージングのための遺伝的にコードされたRNAベースのCHA回路を開発する.
- 合成核酸回路の限界を克服して 長期にわたる細胞応用に
- プログラム可能なシステムを設計して 様々なRNAの標的をイメージする
主な方法:
- 遺伝的にコードされたRNAベースの 触媒式ヘアピン組立回路を利用した
- ブロッコリーRNAアプタマーの分割版をフローロゲンレポーターとして使用した.
- 様々な標的RNAの配列をイメージするための回路を設計した.
主要な成果:
- 生体細胞内の標的RNAの 敏感な検出を達成した.
- 一つの標的RNAが複数のブロッコリーアプタマーから触媒的に光を引き起こすことが示された.
- 汎用的なRNAイメージングアプリケーションのためのプログラム可能なシステムを開発しました.
結論:
- 遺伝的にコードされたRNA回路は 細胞の長期的分析と調節のための強力な代替手段です
- 開発されたRNAベースのCHA回路は,生体細胞における敏感でプログラム可能なRNAイメージングを可能にします.
- この設計原理は,細胞アプリケーションのための多様なRNA回路の作成を容易にする.
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