覚醒したマウスの網膜から単ニューロンの慢性記録を行う方法
Guosong Hong1, Tian-Ming Fu1, Mu Qiao2
1Department of Chemistry and Chemical Biology, Harvard University, Cambridge, MA, USA.
Science (New York, N.Y.)
|June 30, 2018
まとめ
研究者は,マウスにおける網膜のギャングリオン細胞 (RGCs) の安定した長期的なインビボ記録のための非外科的方法を開発した. この発見により 覚醒している動物の神経回路と視覚処理を 詳細に研究することが可能になりました
科学分野:
- 神経科学
- 眼科について
- 生物医学工学
背景:
- 網膜は 神経回路の研究の重要なモデルです
- 網膜の慢性 in vivo 電気生理学は困難でした
- 以前の方法は安定性や侵襲性が限られていた.
研究 の 目的:
- 網膜のギャングリオン細胞 (RGC) の安定した慢性 in vivo 電気生理学のための非外科的方法を開発する.
- 覚醒して行動している動物のRGCの長期監視を可能にします.
- 神経回路の機能と 網膜の昼夜リズムを調査する
主な方法:
- 新しい非同軸内視内注射技術が開発された.
- 網膜の電子メッシュは 網膜を展開し 整合的に覆うように設計されています
- 覚醒したマウスで16チャネルの電気生理学的記録が行われました.
主要な成果:
- この方法により,単一のRGCから2週間以上の長期的に安定したインビボ記録が得られました.
- 視覚刺激に対する 安定した反応を記録した
- RGCの反応における昼夜リズムは,複数の昼夜サイクルで観察されました.
結論:
- メッシュエレクトロニクスの非外科的内視内注射は,慢性 in vivo 網膜記録のための安定したプラットフォームを提供します.
- この技術は,網膜の神経回路の研究における以前の制限を克服します.
- 視覚の処理と 網膜の昼夜調節の理解に 新たな道を開きます
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