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Preparation of High-Temperature Sample Grids for Cryo-EM
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ミトコンドリアカルシウムユニポーターの真菌およびメタゾアンの冷凍-EM構造
Rozbeh Baradaran1, Chongyuan Wang1, Andrew Francis Siliciano1,2
1Structural Biology Program, Memorial Sloan Kettering Cancer Center, New York, NY, USA.
Nature
|July 12, 2018
まとめ
ミトコンドリアのカルシウムユニポーター (MCU) チャンネル構造は,冷凍-EMによって明らかにされ,
科学分野:
- バイオ物理学
- 分子生物学
- 構造生物学
背景:
- ミトコンドリアカルシウムユニポーター (MCU) は,ミトコンドリアへのカルシウム流入を調節する重要な経路である.
- MCUのイオン浸透と選択性メカニズムを理解することは極めて重要ですが,そのユニークなアーキテクチャのために困難です.
研究 の 目的:
- 異なる種からのMCUチャンネルの高解像度構造を決定する.
- カルシウムイオンの選択性とMCUチャネル経由の浸透の分子基礎を解明する.
主な方法:
- ゼブラフィッシュとCyphellophora europaea MCUの冷凍電子顕微鏡 (冷凍EM) 再構築
- 高解像度 (3.2 Å) のC. europaea MCUの原子モデル
主要な成果:
- ゼブラフィッシュとC. europaeaの両方のMCUチャネルは,前回の報告とは異なるテトラメリックステキオメトリを採用しています.
- 保存されたWDXXEPモチーフは選択性フィルターを形成し,カルシウムイオンの単一ファイルの配置を可能にします.
- 異なったN端ドメイン組成 (C. europaeaのディマー,ゼブラフィッシュの非対称な半月) が観察された.
結論:
- 決定された構造は,MCUチャネルのテトラメリックな性質を明らかにする.
- WDXXEPの選択性フィルターとN末端ドメイン組織は,カルシウムイオン浸透性と選択性についての洞察を提供します.
- これらの発見は,MCUのチャネル機能を理解するための構造的基礎を提供します.
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