マウス染色体11のホメオボックスの遺伝子複合体: 分子クローン,胚形成における発現,ヒトのホメオボックスの位置への同質性

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C P Hart, A Awgulewitsch, A Fainsod

|November 1, 1985

まとめ

哺乳類のホメオボックスの配列は,胚の発達に極めて重要です. この研究では,マウスのホックス-2の位置を特定し,ドロソフィラに似た保存された遺伝子組織と発現パターンを明らかにしました.

科学分野:

発達生物学発達生物学とは
遺伝学遺伝学とは
分子生物学は分子生物学である.

背景:

ホメオボックスの配列は,ホメオ遺伝子のタンパク質をコードする保存ドメインである.
哺乳類のホメオボックスの配列は,胚のパターン形成を制御すると仮定されています.

研究の目的:

哺乳類のホメオボックス配列が胚形成における役割を調査する.
マウスをクローンし,マウスのHox-2ロカスとそのトランスクリプトを特徴づける.

主な方法:

重複する複合ファグクローンのクローニング.
マウス染色体11のHox-2ロクスを含む領域の特徴.
胚形成中のトランスクリプト発現の分析.

主要な成果:

マウス染色体11の4つのホメオボックス (Hox-2ロカス) のクラスタを特定しました.
Hox-2ローカスは,胚形成中に発現する複数のトランスクリプトをコードする.
マウスのホックス-2領域と人間のホックス-2場所の間の広範なホモロジーを発見し,組織が保存されました.

結論:

直接的な証拠は,胚の発達におけるホメオボックス遺伝子の役割を支持しています.
マウスのHox-2ロカスには,ドロソフィラに似た,保存された組織と複雑なトランスクリプト表現が表れています.
発見は,種間のパターン形成のための保存されたメカニズムを示唆しています.

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