トランスジェニックドライバーとレポーターマウスラインのスイート 強化された脳細胞タイプターゲティングと機能
Tanya L Daigle1, Linda Madisen1, Travis A Hage1
1Allen Institute for Brain Science, Seattle, WA 98109, USA.
Cell
|July 16, 2018
まとめ
研究者は脳細胞の正確な研究のために 新しい変異性マウスラインを開発しました TIGRE2.0プラットフォームは,遺伝子ターゲティングのための高度なツールを提供し,ニューロン集団のより良い識別と操作を可能にします.
科学分野:
- 神経科学
- 遺伝学
- 分子生物学
背景:
- 脳細胞の機能を理解するには 遺伝的ツールが不可欠です
- 既存のトランスジェニックマウス系は,特異性と発現レベルに制限があります.
研究 の 目的:
- 新しいドライバーとレポーター トランスジェニックマウスラインを報告する.
- マウスの脳に遺伝的標的を 強化するためのTIGRE2.0トランスジェニックプラットフォームを導入する.
主な方法:
- 皮質と皮質下細胞の集団のための23の新しいドライバラインの開発.
- クレア依存リポーターを含む様々な分子ツールで26の新しいリポーターラインの作成.
- TIGRE2.0プラットフォームを利用して単複製標的挿入トランスジェネシスを行う.
主要な成果:
- TIGRE2.0プラットフォームは,多様なニューロンタイプで高レベルのトランスゲン発現を可能にします.
- 新しい線は,光学的生理学,光遺伝学,特定の細胞集団の希少なラベル付けを容易にする.
- 過去のTIGREラインと比較して単純化された育種戦略
結論:
- マウスの脳細胞の 精度の高い遺伝子操作のための ツールキットを大幅に拡張します
- これらのツールは 異なるニューロンの集団を特定し 監視し 制御する研究を加速します
- TIGRE2.0プラットフォームは,神経科学の研究のための遺伝子工学の重要な進歩を表しています.
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