高度な秩序とフィールドフリー3DDNAナノ構造:急速な単段階感知のための次世代DNAナノマシン
Pu Zhang1, Jie Jiang1, Ruo Yuan1
1Key Laboratory of Luminescent and Real-Time Analytical Chemistry (Southwest University), Ministry of Education, School of Chemistry and Chemical Engineering , Southwest University , Chongqing 400715 , People's Republic of China.
Journal of the American Chemical Society
|July 17, 2018
まとめ
研究者はアゾベンゼンで機能したDNAナノマシンを開発しました このDNAナノ構造は バイオセンシングのアプリケーションで 迅速なバイオマーカー検出のための 効率的で可逆的な動きを示しています
科学分野:
- ナノテクノロジー
- バイオテクノロジー
- 分子工学
背景:
- 伝統的なナノマシンは 効率性と可逆性において 課題に直面しています
- DNAナノテクノロジーは 精密な自己組み立て能力を提供します
- アゾベンゼン誘導体は 光に反応する分子制御を可能にします
研究 の 目的:
- フィールドフリーで自己組み立ての 3D DNA ナノマシンを開発するために
- バイオセンシングのための 効率的で可逆的な移動を 達成するためです
- バイオマーカーの迅速な検出を証明する
主な方法:
- 3Dナノ構造に 自己組み立てアゾベンゼン機能化DNAナップパー
- 構造の整合性を確保するために ワトソン・クリック・ベース・ペアリングを使用しています
- リバーシブルなニッパー活動と移動のためのアゾベンゼンの光同化を使用します.
主要な成果:
- フィールドフリー3DDNAナノ構造が 急速に組み立てられました
- DNAナノマシンは 従来のデザインよりも 動きの効率が向上しました
- リバーシブルな移動と単段階のバイオマーカーの検出は10分以内に実証されました.
結論:
- 開発された3DDNAナノマシンは 逆転可能な移動のための新しいプラットフォームを提供します
- この技術はDNAナノテクノロジーと バイオセンシングの 重要な課題を克服しています
- この発見は 診断や検出の先端の 機械装置の道を開きます
関連する概念動画
DNA-only Transposons
17.5K
DNA-only transposons are called autonomous transposons since they code for the enzyme transposase that is required for the transposition mechanism. Insertion of transposons can alter gene functions in multiple ways. They can mutate the gene, alter gene expression by introducing a novel promoter or insulator sequence, introduce new splice sites, and change the mRNA transcripts produced, or remodel chromatin structure.
The donor site from where the transposon is excised is either degraded or...
The donor site from where the transposon is excised is either degraded or...
17.5K
DNA Helicases
24.2K
DNA unwinding helicase enzymes are a type of motor protein. Motor proteins can translocate along filaments or polymers using energy generated from ATP hydrolysis. Helicases are involved in all the important cellular processes where DNA unwinding is required, such as DNA replication, repair, recombination, and transcription. They are present in all living organisms, but vary in their structure, function, and mechanism of action. For example, in prokaryotes, DnaB helicase binds and translocates...
24.2K
DNA Topoisomerases
35.7K
Topoisomerases are enzymes that relax overwound DNA molecules during various cell processes, including DNA replication and transcription. These enzymes regulate positive and negative DNA supercoiling without changing the nucleotide sequence. DNA overwinding in a clockwise direction results in positively supercoiled DNA, whereas underwinding in a counterclockwise direction produces negatively supercoiled DNA.
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
Types and Mechanism of action
Topoisomerases are divided into two main types. ...
35.7K
DNA Replication
59.6K
DNA replication involves the separation of the two strands of the double helix, with each strand serving as a template from which the new complementary strand is copied. After replication, each double-stranded DNA includes one parental or “old” strand and one “new” strand. This is known as semiconservative replication. The resulting DNA molecules have the same sequence and are divided equally into the two daughter cells.
Replication in Prokaryotes
DNA replication...
Replication in Prokaryotes
DNA replication...
59.6K
From DNA to Protein
22.5K
The flow of genetic information in cells from DNA to mRNA to protein is described by the central dogma, which states that genes specify the sequence of mRNAs, which in turn specify the sequence of amino acids making up all proteins. The decoding of one molecule to another is performed by specific proteins and RNAs. Because the information stored in DNA is so central to cellular function, it makes intuitive sense that the cell would make mRNA copies of this information for protein synthesis...
22.5K
Overview of DNA Repair
33.8K
In order to be passed through generations, genomic DNA must be undamaged and error-free. However, every day, DNA in a cell undergoes several thousand to a million damaging events by natural causes and external factors. Ionizing radiation such as UV rays, free radicals produced during cellular respiration, and hydrolytic damage from metabolic reactions can alter the structure of DNA. Damages caused include single-base alteration, base dimerization, chain breaks, and cross-linkage.
Chemically...
Chemically...
33.8K


