豚 の 心臓 の 早期 再生 能力
Lei Ye1, Giuseppe D'Agostino2, Sze Jie Loo1
1National Heart Research Institute Singapore, National Heart Centre Singapore (L.Y., S.J.L., C.X.W., L.P.S., S.H.T., G.Z.T., C.J.P., R.S.T., S.A.C.).
Circulation
|July 22, 2018
まとめ
若い豚の心臓は 心筋細胞の分裂によって 損傷後に再生できます この心臓の再生能力は ゼブラフィッシュやニュートンのように 大きい哺乳類では 誕生直後に失われます
科学分野:
- 心血管生物学
- 再生医療
- 哺乳類の心臓の発達
背景:
- 大人の哺乳類の心臓は 損傷後の自己修復能力が限られています
- 心臓組織再生は,主に心筋細胞 (CM) 増殖によって,斑馬魚,新生児マウスで観察されています.
- 若い大型哺乳類の心臓の 再生の可能性は ほとんど未知のままです
研究 の 目的:
- 新生児の心臓発作後の豚心臓の再生能力を調査する.
- 幼い大型哺乳類の心臓再生に 心筋細胞の分裂が寄与するかどうかを判断する.
主な方法:
- 新生児の豚 (2,3日または14日) は心筋梗塞または偽手術を受けた.
- 心臓の機能と傷跡はMRIとエコーカーディオグラフィーを用いて評価された.
- 細胞増殖,筋細胞分裂マーカー,および線維症は,組織学,免疫ヒスト化学,ウエスタン・ブロッティング,およびRNA配列解析によって分析された.
主要な成果:
- 2日目の豚は機能回復を示し,心臓発作後の繊維症のない壁の厚さを維持しました.
- 高齢の豚 (14日) は大きな傷痕,壁の薄れ,心臓機能の障害を発症した.
- 若い豚の再生した心臓はCMDNA複製と分裂マーカーを示し,古い豚の繊維性心臓とは異なり,細胞運動遺伝子の強化を示した.
結論:
- 新生児の豚の心臓は,心筋細胞の分裂によって再生可能である.
- この再生能力は一時的であり,大型哺乳類では出生後に急速に失われます.
- この再生の窓を理解することで 心臓の損傷の治療戦略を 導き出せます
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