人間のc-erb-B-2遺伝子によってコードされたタンパク質が,表皮成長因子受容体と類似している
Nature
|January 16, 1986
まとめ
EGF受容体と同様のc-erb-B-2遺伝子は,いくつかのがんでは増幅されています. 分子クローニングにより,胃がん細胞に変異したトランスクリプトがある潜在的な受容体タンパク質をコードすることが明らかになった.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 腫瘍学 腫瘍学
背景:
- 人間のゲノムで確認された新しいv-erb-B関連遺伝子,c-erb-B-2は,染色体17q21にマップされています.
- この遺伝子は,表皮成長因子 (EGF) 受容体に同質なキナーゼ領域を持つポリペプチドをコードする.
- c-erb-B-2遺伝子は脊椎動物に保存されており,ネズミのneu遺伝子はその同型であると考えられている.
研究 の 目的:
- c-erb-B-2タンパク質の性質を決定するために.
- EGF受容体とc-erb-B-2タンパク質の類似性を分析する.
- 腫瘍形成過程におけるc-erb-B-2遺伝子増幅と過剰発現の役割を調査する.
主な方法:
- MKN-7細胞からのc-erb-B-2メッセンジャーRNAのための補完DNAの分子クローン.
- クローンされたcDNAの配列分析.
- 癌細胞系におけるc-erb-B-2遺伝子増幅とトランスクリプトレベルの分析.
主要な成果:
- c-erb-B-2遺伝子は,推定受容体タンパク質をコードする.
- 配列分析により,EGF受容体と新製品との類似性が明らかになった.
- 胃がん細胞系 (MKN-7) でのc-erb-B-2の増幅により,正常 (4.6kb) と断片化 (2.3kb) のトランスクリプトの両方の合成が増加しました.
- 断片化されたトランスクリプトは,おそらく受容体の細胞外領域をコードする.
結論:
- c-erb-B-2遺伝子産物は潜在的受容体タンパク質である.
- 増幅と変異したトランスクリプトを含む異常なc-erb-B-2遺伝子発現は,がん発症と関連しています.
- c-erb-B-2タンパク質の機能と腫瘍発生における役割に関するさらなる調査が必要である.
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