32P-イノシトール-1,4,5-トリフォスファートの飽和性受容体で,肝細胞と中性粒子の体内にある
Nature
|February 6, 1986
まとめ
研究者らは,重要なシグナル伝達分子であるイノシトール-1,4,5-トリスホスファート (Ins(1,4,5) P3) の特定の細胞内受容体を特定した. この発見は,細胞のカルシウム (Ca2+) 調節と信号伝達経路の理解を前進させる.
科学分野:
- バイオケミストリー バイオケミストリー
- 細胞生物学 細胞生物学
- 分子薬理学 分子薬理学
背景:
- 受容体の活性化により,ポリフォスフォノシチドの分解が始まり,イノシトール-1,4,5-トリフォスファート (Ins(1,4,5) P3) が生成されます.
- Ins(1,4,5) P3は,細胞内貯蔵物からカルシウム (Ca2+) の放出を媒介する第二メッセンジャーである.
- 以前の研究によると,Ins(1,4,5) P3は特定の細胞内受容体を通して作用し,おそらくはエンドプラズマ網膜に作用する.
研究 の 目的:
- 推定のIns(1,4,5) P3受容体.を特定し,特徴づけること.
- Ins(1,4,5) P3が特定の細胞部位に結合する直接的な証拠を提供するためです.
主な方法:
- 放射性標識32P-Ins ((1,4,5) P3) を使った結合測定法.
- 浸透した豚肝細胞とウサギの中性子細胞で結合研究を行った.
主要な成果:
- 浸透した細胞で32P-Ins ((1,4,5) P3) の特定の飽和結合が実証されています.
- 結合部位の特性を特徴付け,生理学的Ins{1,4,5) P3受容体を表していることを示唆した.
結論:
- 特定された結合部位は,Ins{1,4,5) P3.3の生理学的受容体と一致する性質を示している.
- この発見は,Ca2+シグナル伝達における第2のメッセンジャーとしてのIns(1,4,5) P3の役割を支持しています.
さらに関連する動画
08:07Identification of Inositol Phosphate or Phosphoinositide Interacting Proteins by Affinity Chromatography Coupled to Western Blot or Mass Spectrometry
Published on: July 26, 2019
09:22Absolute Quantitation of Inositol Pyrophosphates by Capillary Electrophoresis Electrospray Ionization Mass Spectrometry
Published on: August 13, 2021
関連する概念動画
What are Second Messengers?
Because many receptor binding ligands are hydrophilic, they do not cross the cell membrane and thus their message must be relayed to a second messenger on the inside. There are several second messenger pathways, each with their own way of relaying information. G-protein coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol path is active when the receptor induces phospholipase C to hydrolyze the phospholipid,...
Phosphoinositides and PIPs
Phosphoinositides are a group of phospholipids containing a glycerol backbone with two fatty acid chains and a phosphate attached to a myoinositol sugar ring. The inositol head group extends into the cytoplasm, where it is modified by adding phosphate groups to form phosphatidylinositol phosphates or PIPs.
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Different phosphoinositides are synthesized and recruited on the cytosolic face of the plasma membrane. The localization of specific phosphoinositides concentrated in separate membrane...
Amplifying Signals via Second Messengers
Many receptor binding ligands are hydrophilic; they do not cross the cell membrane but bind to cell-surface receptors. Thus, their message must be relayed by second messengers present in the cell cytoplasm. There are several second messenger pathways, each with its own way of relaying information. For example, the G protein-coupled receptors can activate both phosphoinositol and cyclic AMP (cAMP) second messenger pathways. The phosphoinositol pathway is active when the receptor induces...
IP3/DAG Signaling Pathway
Membrane lipids such as phosphatidylinositol (PI) are precursors for several membrane-bound and soluble second messengers. Specific kinases phosphorylate PI and produce phosphorylated inositol phospholipids. One such inositol phospholipids are the phosphatidylinositol-4,5 bisphosphate [PI(4,5)P2], present in the inner half of the lipid bilayer. Upon ligand binding, GPCR stimulates Gq proteins to turn on phospholipase Cꞵ. Activated phospholipase Cꞵ cleaves PI(4,5)P2 and produces two-second...
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT)
Insensitive Nuclei Enhanced by Polarization Transfer (INEPT) is an advanced Nuclear Magnetic Resonance (NMR) technique specifically designed to detect and enhance the signals of low-abundance nuclei, such as carbon-13 and nitrogen-15, in small molecules. The fundamental principle behind INEPT is the transfer of polarization from a more abundant and highly polarizable nucleus, typically hydrogen-1, to the low-abundance nucleus of interest. This process effectively boosts the NMR signal of the...
