ヘム・ヘム・エキシトン結合を示す周期構造を持つ超分子ヘモプロテイン組

Koji Oohora^1,2,3, Nishiki Fujimaki¹, Ryota Kajihara¹

¹Department of Applied Chemistry, Graduate School of Engineering , Osaka University , Suita 565-0871 , Japan.

Journal of the American Chemical Society

|August 2, 2018

まとめ

研究者は,サイトクロームb562とヘムを用いて,新しい人工ヘモプロテインを組み立てました. この周期的な螺旋構造はヘム-ヘムポケットの相互作用によって駆動され独特の光学特性を表しています

科学分野:

生物化学
バイオ物理学
材料科学

背景:

サイトクロームb562は小さな血液タンパク質です.
人工のヘモプロテイン・アセンブリは,新しい機能的な材料を作るのに興味があります.
タンパク質の構造を設計する際には分子間相互作用を理解することが重要です

研究の目的:

細胞染色体b562単位と外部に付着したヘムの明確に定義された超分子組成を構成する.
ヘモプロテイン組の構造と光学的性質を調査する
アセンブリ・フォーメーションの背後にある推進力を明らかにする

主な方法:

表面ヘム結合のためのサイトクロームb562のN80C変異体の設計.
円形二極光学 (CD) と高速原子力顕微鏡 (AFM) を用いた特徴化.
分子ダイナミクスシミュレーション,シミュレートされたCDスペクトル,AFM画像,変異実験による検証

主要な成果:

シトクロームb562の周期性上分子組成の成功
ヘム-ヘムエクシトン結合による分割型コットン効果の観測,人工ヘモプロテイン系では初めて.
3nmのピッチを持つ周期的な螺旋構造の決定.

結論:

エンジニアリングされたN80C変異体は周期組成の形成を促進します
ヘム-ヘムポケットの相互作用は,二次的な水素結合および/または静電相互作用によって補完された主要な駆動力である.
この研究は,ユニークな構造と光学特性を有する新種の人工血液タンパク質を提示します.

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