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Updated: Feb 7, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
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Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

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タンパク質半合成を用いたアクトキナーゼ活性化メカニズム

Nam Chu1, Antonieta L Salguero1, Albert Z Liu2

  • 1Division of Genetics, Department of Medicine, Brigham and Women's Hospital, Boston, MA 02115, USA; Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115, USA; Department of Pharmacology and Molecular Sciences, Johns Hopkins School of Medicine, Baltimore, MD 21205, USA.

Cell
|August 7, 2018
PubMed
まとめ

リン酸化によるアクトタンパク質の活性化には,異なるメカニズムが含まれています. Ser477 / Thr479での二重リン酸化は,Akt活性とPIP3結合を調節するために異なる経路を使用します.

キーワード:
X線結晶構造二基板類型タンパク質の結合キナーゼマススペクトロメトリーペプチドリン酸化フォトクロスリンク

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