CRISPRによるマウス全体の発達バーコード

Reza Kalhor^1,2, Kian Kalhor³, Leo Mejia⁴

¹Department of Genetics, Harvard Medical School, Boston, MA, USA. gchurch@genetics.med.harvard.edu kalhor@genetics.med.harvard.edu.

Science (New York, N.Y.)

|August 11, 2018

まとめ

CRISPR技術を使ってマウスの細胞の歴史を記録する新しいバーコードシステムを開発しましたこの方法により精密な系統追跡と哺乳類の発達研究が可能です

科学分野:

発達生物学
遺伝学
分子生物学

背景:

生体内のバーコードは,発達系を含む生物学的情報を理解するために不可欠です.
哺乳類のシステムにおけるヌクレアース誘発変異バーコードの以前の応用は限られていた.
CRISPRベースの系統追跡は高解像度の生物学的記録の可能性を秘めています

研究の目的:

マウスで多用途のバーコードプラットフォームを確立する.
哺乳類の系統追跡と発達研究を可能にします
初期の発達系統を再構築し脳軸の発達を調べる

主な方法:

CRISPR媒介による in vivo 変異を誘導するマルチホーミングガイドRNAを使用した.
妊娠時にバーコードが活性化され,妊娠中の突然変異が継続される.
組み合わせた変異で指数関数的に多様なバーコードを生成した.
初期の血統の再構築と脳の発達分析のための適用されたバーコード.

主要な成果:

系統に特異的な変異を持つ発達的にバーコード化されたマウスを成功裏に生み出した
最古の哺乳類の系統を再構築した.
脳軸形成の発達過程の調査を可能にしました.
高解像度の系統追跡のプラットフォームの能力を実証しました

結論:

開発された in vivo バーコードシステムは,哺乳類の研究のための可能で汎用的なプラットフォームです.
この技術はマウスモデルの系統追跡能力を大幅に向上させます
このシステムは発達生物学と病気のメカニズムを研究するための新しい道筋を提供します

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