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Whole Cell Recordings from Brain of Adult Drosophila
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成人マウスの脳細胞の分子多様性と特異性
Arpiar Saunders1, Evan Z Macosko2, Alec Wysoker1
1Department of Genetics, Harvard Medical School, Boston, MA 02115, USA; Stanley Center for Psychiatric Research, Broad Institute of MIT and Harvard, Cambridge, MA 02142, USA.
Cell
|August 11, 2018
まとめ
この研究では 69万個のネズミの脳細胞をマッピングし 565の異なる細胞群を特定しました この発見は脳の組織と 神経細胞の特異性を明らかにし 価値ある細胞アトラスを生み出しました
科学分野:
- 神経科学
- ゲノミクス
- コンピュータ生物学
背景:
- 哺乳類の脳には 多くの特殊な細胞タイプがあります
- 細胞の多様性を理解することは 神経科学の研究に不可欠です
研究 の 目的:
- 成人マウスの脳内の個々の細胞のRNA発現を体系的にプロファイルする.
- セルアトラスを作成し,さらに研究する.
主な方法:
- 69万個の細胞のRNA発現をプロファイルするためにDrop-seqを使用した.
- 565の転写的に異なる細胞群を特定するための計算方法が適用されました.
- 成人マウスの脳の9つの領域の 細胞集団を分析した
主要な成果:
- 大人のマウスの脳で 565の異なる細胞群を特定しました
- アクソナルとプレシナプスの遺伝子発現モジュールを明らかにした.
- ボルテージゲートイオンチャネルの共同展開と血管細胞機能のパターンを発見した.
- グルタマタージックニューロンの特異化と希少な棘状投影ニューロンを特定した.
結論:
- 成人マウスの脳の詳細な細胞アトラスを提供しています.
- このアトラスは脳の組織や ニューロンの専門化 細胞の多様性についての洞察を 提供しています
- DropVizソフトウェアは,開発,病気,進化の研究のためのこの貴重なリソースのインタラクティブ探索を可能にします.
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