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Cell Diversity01:13

Cell Diversity

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The concept of a cell started with microscopic observations of dead cork tissue by Robert Hooke in 1665. Hooke coined the term "cell" based on the resemblance of the small subdivisions in the cork to the rooms that monks inhabited, called cells. About ten years later, Antonie van Leeuwenhoek became the first person to observe the living and moving cells under a microscope. In the century that followed, the theory that cells represented the basic unit of life developed.
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Diversity in Cell Signaling Responses01:22

Diversity in Cell Signaling Responses

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The physiological function of a cell and cellular communication are outcomes of a range of extrinsic signals, intracellular signaling pathways, and cellular responses. No two cell types express the same repertoire of signaling components. Receptors are highly selective for their cognate ligands, but once activated, they can alter multiple cellular processes such as DNA transcription, protein synthesis, and metabolic activity. 
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Diversity of Archaea I01:30

Diversity of Archaea I

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Archaea, a domain of single-celled microorganisms, are classified into five major phyla based on genetic and biochemical characteristics: Euryarchaeota, Crenarchaeota, Thaumarchaeota, Korarchaeota, and Nanoarchaeota. Among these, the phylum Euryarchaeota is notable for its remarkable diversity in morphology, metabolism, and ecological adaptations.Morphological and Metabolic DiversityMembers of Euryarchaeota exhibit a variety of cellular shapes, including rods and cocci. Their metabolic pathways...
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Diversity of Archaea II01:24

Diversity of Archaea II

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Archaea, one of the three domains of life, exhibit remarkable diversity and adaptability, thriving in both extreme and moderate environments. Historically, most identified archaea have been classified into two major phyla: Euryarchaeota and Crenarchaeota. However, recent molecular studies have expanded this classification to include three additional phyla: Thaumarchaeota, Nanoarchaeota, and Korarchaeota, each exhibiting unique characteristics and ecological roles.Thaumarchaeota: Mesophiles...
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Diversity of Protists I01:15

Diversity of Protists I

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Excavata is a diverse group of protists that includes both chemoorganotrophic and phototrophic species, with some thriving in anaerobic environments. Among the key groups within Excavata are diplomonads and parabasalids, which are flagellated protists that lack mitochondria and chloroplasts. These microorganisms typically inhabit anoxic environments, such as the intestines of animals, where they exist either symbiotically or as parasites, relying on fermentation for energy production. Some...
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Diversity of Protists II01:27

Diversity of Protists II

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Alveolates are a group of organisms recognized by the presence of alveoli, which are cytoplasmic sacs located beneath the cell membrane. While their function remains uncertain, alveoli may help regulate water balance by controlling how much water enters and leaves the cell. In dinoflagellates, these structures may serve as armor plates. There are three major types of alveolates: ciliates, which move using cilia; dinoflagellates, which use flagella for movement; and apicomplexans, which are...
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T and B Cell Receptor Immune Repertoire Analysis using Next-generation Sequencing
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多様性 を 一度に 一つの 細胞 で 配列化 する

Michael C Oldham1, Anatol C Kreitzer2

  • 1Department of Neurological Surgery, UCSF, San Francisco, CA 94143, USA; Weill Institute for Neurosciences, UCSF, San Francisco, CA 94158, USA.

Cell
|August 11, 2018
PubMed
まとめ
この要約は機械生成です。

単細胞RNA配列解析は 細胞の多様性に関する新しい洞察を 提供しています 3つの研究で この技術を使って マウスとハエの神経系に 異なる細胞タイプをマッピングしました

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科学分野:

  • 神経科学
  • ゲノミクス
  • 細胞生物学

背景:

  • 複雑な生物学的システムを研究するには 細胞の多様性を理解することが重要です
  • 伝統的な方法は 細胞の全スペクトルを捉えるのに苦労します

研究 の 目的:

  • 単細胞RNA配列を大規模に適用する.
  • 神経系内の異なる細胞集団を特定し特徴づけること.

主な方法:

  • 単細胞RNAシーケンシング (scRNA-seq) を利用する.
  • 個々の細胞の転写プロフィールを分析する.
  • ネズミとハエの神経系を 比較しています

主要な成果:

  • 細胞の種類を区別する 新規の転写パターンを明らかにした.
  • 神経系の細胞多様性の 高解像度マップを提供した.
  • 特定の遺伝子発現シグネチャーを特定した. 異なる神経細胞と膠質細胞.

結論:

  • 単細胞RNAシーケンシングは 細胞の異質性を解剖する強力なツールです
  • これらの発見は 神経系の発達と機能の理解を 進めてくれます
  • この研究は,細胞特異的なメカニズムに関する将来の研究のための基盤を確立します.