周周血液リンパ球と腫瘍オルガノイドの共同培養による腫瘍反応性T細胞の生成

Krijn K Dijkstra¹, Chiara M Cattaneo¹, Fleur Weeber¹

¹Department of Molecular Oncology and Immunology, the Netherlands Cancer Institute, Antoni van Leeuwenhoek Hospital, 1066 CX Amsterdam, the Netherlands.

Cell

|August 14, 2018

まとめ

研究者は個々のガンT細胞の反応を研究するための新しいプラットフォームを開発しましたこの方法は,上皮がん患者の腫瘍反応性T細胞を増やし,免疫療法の研究を支援します.

科学分野:

腫瘍学
免疫学
バイオテクノロジー

背景:

癌の免疫療法には希望がありますがパーソナライズされた研究プラットフォームは不足しています
T細胞の相互作用を理解することは,上皮がんの治療反応を予測するのに極めて重要です.

研究の目的:

腫瘍特異のT細胞反応を研究するための個別化されたプラットフォームを確立し,検証する.
T細胞の相互作用を分析し,免疫療法への反応の決定要因を特定する.

主な方法:

周周血液リンパ球と自己組織腫瘍を共培養する.
腫瘍反応性T細胞の増殖適合不良の結腸直腸がんと非小細胞肺がんの患者
濃縮されたT細胞とマッチした腫瘍オルガノイドの殺戮効率の評価

主要な成果:

パーソナライズされたT細胞応答分析のプラットフォームを成功裏に確立し,検証しました.
腫瘍反応性T細胞の濃縮が示されました
T細胞による腫瘍破壊の効率を評価する能力を示した

結論:

開発されたプラットフォームは,腫瘍反応性T細胞の偏らない分離を可能にします.
T細胞攻撃に対する個々の患者の腫瘍の感受性を評価する方法を提供します.
パーソナライズされたがん免疫療法の研究を進めるための新しい戦略を提示します

キーワード:

T細胞アドプティブ・セル・転送大腸がん免疫チェックポイントの封鎖免疫療法マイクロサテライト不安定不一致修理欠陥非小細胞肺がんオーガノイド

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