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昆虫の嗅覚受容体Orcoの冷凍-EM構造
Joel A Butterwick1, Josefina Del Mármol1, Kelly H Kim2
1Laboratory of Neurophysiology and Behavior, The Rockefeller University, New York, NY, USA.
Nature
|August 17, 2018
まとめ
昆虫の嗅覚受容体 (Orco) は,さまざまな環境化学物質を検出するために新しいチャネル構造を使用します. このオルコーホモメルの構造的な洞察は 嗅覚システムの進化と 匂いの調節を理解するのに役立ちます
科学分野:
- 生物化学
- 構造生物学
- 神経科学
背景:
- 昆虫は,保存された共受容体 (Orco) と可変の嗅覚受容体を含む環境化学物質を検出するために嗅覚受容体 (ORs) に依存しています.
- オルコ共受容体は嗅覚信号伝達に不可欠であり,殺虫剤の標的である.
研究 の 目的:
- オーコホモアの高解像度構造を 決定するために
- オルコ受容体家族と 嗅覚の差別におけるその役割について 構造的な洞察を提供すること.
主な方法:
- 単粒子の冷凍電子顕微鏡 (Cryo-EM) を用いて,オルコホモールの構造を解析した.
- 構造は3.5 Åの解像度で決定された.
主要な成果:
- 4つのサブユニットが中央の孔を形成し,横の管に分岐しています.
- オルコ・テトラメアは細胞膜内のサブユニット間の相互作用が限られており,細胞膜のアンカードメインによって安定しています.
- ORにおけるシーケンス保存は孔とアンカー領域に集中し,建築の柔軟性を説明する.
結論:
- ORファミリー内のシーケンスの多様性に対応するユニークなチャネルアーキテクチャを明らかにします.
- この構造的な理解は 匂いを調節するメカニズムと 昆虫の嗅覚の進化の研究を容易にする.
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