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Updated: Feb 6, 2026

Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects
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Bio-layer Interferometry for Measuring Kinetics of Protein-protein Interactions and Allosteric Ligand Effects

Published on: February 18, 2014

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ビリオンナノ振動器を用いた膜タンパク質へのリガンド結合運動の測定

Guangzhong Ma1, Guan-Da Syu2,3,4, Xiaonan Shan1,5

  • 1Biodesign Center for Bioelectronics and Biosensors , Arizona State University , Tempe , Arizona 85287 , United States.

Journal of the American Chemical Society
|August 17, 2018
PubMed
まとめ

研究者は,リガンドが膜タンパク質に結合する方法を測定するために,ビリオン振動器を用いた新しい方法を開発しました. このラベルフリー技術は 薬剤発見と細胞機能研究のための 精密なリアルタイム運動測定を提供します

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関連する概念動画

Ligand Binding Sites02:40

Ligand Binding Sites

Proteins are dynamic macromolecules that carry out a wide variety of essential processes; however, the activities of most proteins depend on their interactions with other molecules or ions, known as ligands.
Protein-ligand interactions are quite specific; even though numerous potential ligands surround a cellular protein at any given time, only a particular ligand can bind to that protein. Moreover, a ligand binds only to a dedicated area on the surface of the protein, known as the...
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Allosteric proteins have more than one ligand binding site; the binding of a ligand to any of these sites influences the binding of ligands to the other sites. When a protein is allosteric, its binding sites are called coupled or linked.  In the case of enzymes, the site that binds to the substrate is known as the active site and the other site is known as the regulatory site. When a ligand binds to the regulatory site, this leads to conformational changes in the protein that can influence...
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Protein-drug binding refers to the interaction between drugs and proteins within the body. This binding process can occur intracellularly, involving drug interactions with enzymes or receptors within cells, or extracellularly, involving plasma proteins in the blood.
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The Equilibrium Binding Constant and Binding Strength

The equilibrium binding constant (Kb) quantifies the strength of a protein-ligand interaction. Kb can be calculated as follows when the reaction is at equilibrium:
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