異質核リボ核タンパク質Hの準RNA認識モチーフの間のリンク器によってコードされた微分構成ダイナミクス
Srinivasa R Penumutchu1, Liang-Yuan Chiu1, Jennifer L Meagher2
1Department of Chemistry , Case Western Reserve University , Cleveland , Ohio 44106 , United States.
Journal of the American Chemical Society
|August 21, 2018
まとめ
異質な核リボ核タンパク質H (hnRNP H) のリンカープロリンはコンパクトな構造を好み,hnRNP Fのアラニンはより柔軟な構造を可能にします. この違いはタンパク質の動態に影響を与え,hNNP F/Hファミリーの機能的多様性を説明する可能性がある.
科学分野:
- 分子生物学
- 構造生物学
- 生物化学
背景:
- 異質核リボ核タンパク質 (hnRNP) F/Hタンパク質は,RNA代謝に関与する重要なRNA結合タンパク質である.
- これらのタンパク質は類似したRNA配列を好みながらも 異なる細胞機能を示す.
- 彼らのモジュールドメイン構造には,タンデムN端準RNA認識モチーフ (qRRMs) とC端qRRM3が含まれています.
研究 の 目的:
- 特定のリンクアミノ酸 (位置105のプロリン) が hnRNP HN端領域 (HqRRM1,2) の構造動態に与える影響を調査する.
- hnRNP H と hnRNP F N 端末ドメインの構造特性を比較する.
主な方法:
- X線結晶学
- カー・パーセル・メイブーム・ギル (CPMG) リラクゼーション分散を含む核磁気共振 (NMR) スペクトロスコーピー
- 小角X線散射 (SAXS)
主要な成果:
- HqRRM1,2ドメインは,コンパクト状態と拡張状態の間の構成均衡に存在する.
- コンパクトなHqRRM1,2構造は,qRRM1-qRRM2インターフェイスに電位面を備えています.
- hnRNPのFN末端領域 (FqRRM1,2) は,HqRRM1,2と比較して,主により拡張され,柔軟な形状を採用しています.
- HqRRM1,2のP105A変異は,より拡張された構造にダイナミクスをシフトさせた.
結論:
- リンカー組成は hnRNP F/H タンパク質の構造力学に大きな影響を与える.
- hnRNP H と F の間の観察された構造的差異は,RNA代謝におけるそれらの異なる機能的役割に寄与する可能性がある.
- hnRNP Hの位置105のプロリンは,よりコンパクトな形状を維持するために重要です.
関連する概念動画
Conformity
48.2K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
48.2K
Nuclear Export of mRNA
8.8K
Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
8.8K
RNA Splicing
60.7K
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
60.7K
Eukaryotic RNA Polymerases
27.1K
RNA Polymerase (RNAP) is conserved in all animals, with bacterial, archaeal, and eukaryotic RNAPs sharing significant sequence, structural, and functional similarities. Among the three eukaryotic RNAPs, RNA Polymerase II is most similar to bacterial RNAP in terms of both structural organization and folding topologies of the enzyme subunits. However, these similarities are not reflected in their mechanism of action.
All three eukaryotic RNAPs require specific transcription factors, of which the...
All three eukaryotic RNAPs require specific transcription factors, of which the...
27.1K
Non-LTR Retrotransposons
13.4K
As the name suggests, non-LTR retrotransposons lack the long terminal repeats characteristic of the LTR retrotransposons. Additionally, both LTR and non-LTR retrotransposons use distinct mechanisms of mobilization. Non-LTR retrotransposons are further divided into two classes - Long interspersed nuclear elements (LINEs) and short interspersed nuclear elements (SINEs), both of which occur abundantly in most mammals, including humans. Some of the active non-LTR retrotransposons in humans are L1...
13.4K
Encoding
867
Information enters the brain through encoding, which is the input of information into the memory system. Once sensory information is received from the environment, the brain labels or codes it. The information is then organized with similar information and connected to existing concepts. Encoding occurs through automatic processing and effortful processing.
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
Automatic processing involves the encoding of details like time, space, frequency, and the meaning of words, usually done without conscious...
867


