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Colloids03:22

Colloids

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Children at play often make suspensions such as mixtures of mud and water, flour and water, or a suspension of solid pigments in water known as tempera paint. These suspensions are heterogeneous mixtures composed of relatively large particles that are visible to the naked eye or can be seen with a magnifying glass. They are cloudy, and the suspended particles settle out after mixing. On the other hand, a solution is a homogeneous mixture in which no settling occurs and in which the dissolved...
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In mechanical engineering, the stability of systems under various forces is critical for designing durable and efficient structures. One fundamental way to explore these concepts is by analyzing systems like two rods connected at a pivot point, O, with a torsional spring of spring constant k at the pivot point. This system is similar in appearance to a scissor jack used to change tires on a car. In this case, the arms of the linkage (equivalent to the rods in this system) are entirely vertical,...
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Overview
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ATP-binding cassette or ABC transporter is the largest superfamily of integral membrane proteins. The transporters have transmembrane-binding domains (TMDs) and nucleotide-binding domains (NBDs). The TMDs are specific to their substrates, whereas the NBDs are similar to engines that complete ATP hydrolysis to complete the substrate transport. They can be full transporters consisting of two TMDs and NBDs, half transporters with one TMD and NBD, while some encoded with a single TMD or NBD are...
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Each human somatic cell contains 6 billion base pairs of DNA. Each base pair is 0.34 nm long, meaning each diploid cell contains a staggering 2 meters of DNA. This long DNA strand is packed inside a nucleus measuring only 10-20 microns in diameter with the help of specialized DNA-binding proteins called histones. Together they form a compact DNA-protein complex called chromatin. The chromatin is further compacted into higher-order structures. The highest level of compaction is achieved during...
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Each human somatic cell contains 6 billion base-pairs of DNA. Each base-pair is 0.34 nm long, which means that each diploid cell contains a staggering 2 meters of DNA. How is such a long DNA strand packed inside a nucleus measuring only 10 - 20 microns in diameter? 
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ポリメリックABCミセルによるpDNAの包装は,コロイドの安定性と構造制御を同時に達成する.

Yaming Jiang1, Timothy P Lodge1,2, Theresa M Reineke2

  • 1Department of Chemical Engineering & Materials Science , University of Minnesota , 421 Washington Avenue SE , Minneapolis , Minnesota 55455 , United States.

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まとめ
この要約は機械生成です。

充電されたナノ粒子を用いたDNA圧縮は 治療の鍵です 研究者らは,ポリメリックミセルにポリエチレングリコール (PEG) コロナを加えると,DNA複合体の安定性が著しく改善され,ミセルプレックス構造が制御され,生物模倣クロマチンのような形が形成されたことを発見した.

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科学分野:

  • 材料科学
  • バイオテクノロジー
  • ポリマー化学

背景:

  • 逆電荷のナノ粒子によるDNA圧縮は,新しい治療薬の開発に不可欠です.
  • インターポリエレクトロライト複合体の組成と構造を制御することは,様々な材料の用途に不可欠です.

研究 の 目的:

  • プラズミドDNAとポリメリックカチオンミセル (AB型とABC型) の複合性を調査する.
  • 外部非イオンコロナ (ポリエチレングリコール - PEG) の長さがミセルプレックスのコロイドの安定性,サイズ,組成,構造にどのように影響するか調べる.
  • 制御されたDNA凝縮のためのミセル構造とミセルプレックス特性との関係を理解する.

主な方法:

  • アンフィフィリックブロックポリマーの自己組み立ては,異なるPEGコロナ長 (0〜10 kDa) の球状ミセルになります.
  • タービディメトリック定位を用いたコロイド安定性の評価
  • 動的および静的光散乱, ζ-ポテンシャル測定,および冷凍伝送電子顕微鏡 (冷凍TEM) を使用してミセルプレクスの特徴付け.

主要な成果:

  • DNA-ミセル複合体 (ミセル複合体) のコロイド安定性は,2 kDaでもPEGコロナを加えると劇的に改善された.
  • ミセルプレックスのサイズ,成分,構造は,PEGブロックの分子量と強く相関していました.
  • ミセルプレックスは,PEGの長さが増加するにつれて,複合体あたりのミセル数が少なく,クロマチンに似たバイオミメティックな"串上のビーズ"の形態を採用した.

結論:

  • ポリケーション性ミセルはDNAを生物模倣構造に効果的に凝縮する.
  • PEGコロナ長さの調整は,ミセルプレックス特性を制御するためのシンプルで強力な戦略を提供します.
  • この研究は,核酸の複合化と結果として生じる複合体に対するミセル構造の効果に関する機械的洞察を提供します.