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Updated: Feb 5, 2026

Genome Engineering of Primary Human B Cells Using CRISPR/Cas9
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Genome Engineering of Primary Human B Cells Using CRISPR/Cas9

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CRISPR-Cas9核酵素を設計し,ターゲット空間を拡大した

Hiroshi Nishimasu1, Xi Shi2,3, Soh Ishiguro4,5,6

  • 1Department of Biological Sciences, Graduate School of Science, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo 113-0033, Japan. nisimasu@bs.s.u-tokyo.ac.jp nureki@bs.s.u-tokyo.ac.jp.

Science (New York, N.Y.)
|September 1, 2018
PubMed
まとめ

科学者は,リラックスしたNGプロトスペーサー隣接モチーフ (PAM) を認識するために,Cas9酵素変種 (SpCas9-NG) を設計した. これによって,ヒトの細胞内のこれまでアクセス不可能なゲノム位置をターゲットにすることで,ゲノム編集の能力を拡張します.

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