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関連する概念動画

Buffers02:56

Buffers

173.1K
A solution containing appreciable amounts of a weak conjugate acid-base pair is called a buffer solution, or a buffer. Buffer solutions resist a change in pH when small amounts of a strong acid or a strong base are added. A solution of acetic acid and sodium acetate is an example of a buffer that consists of a weak acid and its salt: CH3COOH (aq) + CH3COONa (aq). An example of a buffer that consists of a weak base and its salt is a solution of ammonia and ammonium chloride: NH3 (aq) + NH4Cl...
173.1K
Lagging Strand Synthesis01:59

Lagging Strand Synthesis

61.4K
During replication, the complementary strands in double-stranded DNA are synthesized at different rates. Replication first begins on the leading strand. Replication starts later, occurs more slowly, and proceeds discontinuously on the lagging strand.
There are several major differences between synthesis of the leading strand and synthesis of the lagging strand. 1) Leading strand synthesis happens in the direction of replication fork opening, whereas lagging strand synthesis happens in the...
61.4K
Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K
Buffers: Buffer Capacity01:09

Buffers: Buffer Capacity

2.5K
Buffer capacity is the quantitative measure of a buffer to resist the change in pH. As shown in the following equation, the buffer capacity, denoted by 'beta', is expressed as the number of moles of acid or base needed to change the pH of a one-liter buffer solution by 1 unit. Here, Ca and Cb indicate the number of moles of acid and base, respectively. Note that dpH represents the change in pH.
In the graph, pH is plotted as a function of the number of moles of base (Cb) added to a weak...
2.5K
Fixing Double-strand Breaks02:04

Fixing Double-strand Breaks

14.8K
The double-stranded structure of DNA has two major advantages. First, it serves as a safe repository of genetic information where one strand serves as the back-up in case the other strand is damaged. Second, the double-helical structure can be wrapped around proteins called histones to form nucleosomes, which can then be tightly wound to form chromosomes. This way, DNA chains up to 2 inches long can be contained within microscopic structures in a cell. A double-stranded break not only damages...
14.8K
Buffer Effectiveness02:19

Buffer Effectiveness

55.4K
Buffer solutions do not have an unlimited capacity to keep the pH relatively constant . Instead, the ability of a buffer solution to resist changes in pH relies on the presence of appreciable amounts of its conjugate weak acid-base pair. When enough strong acid or base is added to substantially lower the concentration of either member of the buffer pair, the buffering action within the solution is compromised.
The buffer capacity is the amount of acid or base that can be added to a given volume...
55.4K

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関連する実験動画

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Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
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Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

Published on: September 29, 2011

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DNAストランド・バッファ

Dominic Scalise, Nisita Dutta, Rebecca Schulman

    Journal of the American Chemical Society
    |September 12, 2018
    PubMed
    まとめ
    この要約は機械生成です。

    研究者はDNA濃度を正確に制御するために新しいオリゴヌクレオチドバッファを開発し,DNAベースのシステムの安定的かつ信頼できる動作を可能にしました. これらのバッファは,調整可能なセットポイント,障害に対する抵抗,および迅速な応答時間を提供します.

    さらに関連する動画

    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method

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    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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    関連する実験動画

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    Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography
    13:46

    Separation of Single-stranded DNA, Double-stranded DNA and RNA from an Environmental Viral Community Using Hydroxyapatite Chromatography

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    Strand-Specific Analysis of Proteins at Replicating DNA Strands by Enrichment and Sequencing of Protein-Associated Nascent DNA Method
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    Published on: May 2, 2025

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    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles
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    Self-assembly of Complex Two-dimensional Shapes from Single-stranded DNA Tiles

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    科学分野:

    • 生物化学
    • 化学工学
    • 分子生物学

    背景:

    • 伝統的なバッファシステムは主にヒドロニウム (pH) のようなイオン濃度を調節します.
    • 既存のバッファリングメカニズムは DNA 配列のような複雑な分子を制御する能力に 限界があります

    研究 の 目的:

    • 短いDNA配列 (オリゴヌクレオチド) を制御できる新しいバッファーを導入する.
    • オリゴヌクレオチドバッファの設計とチューニングのための数学的枠組みを提供する.

    主な方法:

    • バッファ特性 (セットポイント,容量,応答時間) を制御するための速度定数を選択するための数学式の開発.
    • 特定のDNA配列と濃度の設計は,望ましい速度常数を達成します.
    • 異なる条件下で安定した濃度を維持するオリゴヌクレオチドバッファーの実験的実証.

    主要な成果:

    • オリゴヌクレオチドバッファは,50〜500nmの干渉にもかかわらず,10〜80nmのセットポイント濃度を成功裏に維持しました.
    • これらのバッファの反応時間は10分未満から1.5時間でした.
    • 交差干渉なしに複数のバッファの並行動作が実証されている.

    結論:

    • オリゴヌクレオチドバッファは,合成生物学と自己組み立てにおけるDNA濃度の安定化のための堅固な方法を提供します.
    • これらのバッファはDNAベースの分子システムの信頼性と持続時間を高めることができます.
    • このバッファリング原理はDNA以外にも 潜在的に他の分子種にも適用できます