内部トランスエステル化によるRNA分裂に対するリボース構成の影響
Fengmin Guo1, Zekun Yue1, Marko Trajkovski2
1State Key Laboratory of Elemento-Organic Chemistry and Department of Chemical Biology, College of Chemistry , Nankai University , Tianjin 300071 , China.
Journal of the American Chemical Society
|September 13, 2018
まとめ
リボースの形状は,内部トランスエステル化中のRNA分裂率に大きく影響する. 南部限定RNAは,ネイティブRNAよりも速い分裂を示し,北部限定RNAは,リボースを強調して,はるかに遅い.
科学分野:
- 生物化学
- 分子生物学
- RNA メタボリズム
背景:
- RNAの分裂は,RNAの処理と代謝の基本的なプロセスです.
- RNAの分裂の調節を理解することは,細胞の機能を理解するために不可欠です.
- 内部トランスエステル化はRNA分裂の鍵となるメカニズムである.
研究 の 目的:
- 内部トランスエステル化によるRNA分裂に対するリボース構成の影響を調査する.
- 特定のリボース構造がRNA分裂率にどのように影響するかを決定する.
主な方法:
- RNAの分裂を研究するために,形状的に制約されたリボヌクレオチドを使用した.
- 異なるリボース構造のRNA分裂率を測定し,比較した.
主要な成果:
- 南部制約リボヌクレオチド > ネイティブリボヌクレオチド ≫ 北部制約リボヌクレオチド
- 南部に制限された形状は,ネイティブRNAと比較して有意に高い割れ目率を示した.
- 北に制限された形状は,割れ目率が大幅に減少した.
結論:
- リボース構成は,内部トランスエステル化によるRNA分裂を調節する上で重要な役割を果たします.
- 特定のリボース形状は,RNAの分裂率を高めたり抑制したりすることができます.
- これらの発見は,RNAの触媒活動の構造的決定因子についての洞察を提供します.
関連する概念動画
01:20Conformity
48.2K
Conformity is the change in a person’s behavior to go along with the group, even if that person does not agree with the group.
48.2K
01:23RNA Structure
79.1K
Overview
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
The basic structure of RNA consists of a five-carbon sugar and one of four nitrogenous bases. Although most RNA is single-stranded, it can form complex secondary and tertiary structures. Such structures play essential roles in the regulation of transcription and translation.
Different Types of RNA Have the Same Basic Structure
There are three main types of ribonucleic acid (RNA): messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). All three RNA types consist of a...
79.1K
01:33Cleavage and Blastulation
50.3K
After a large-single-celled zygote is produced via fertilization, the process of cleavage occurs while zygotes travel through the uterine tube. Cleavage is a mitotic cell division that does not result in growth. With each round of successive cell division, daughter cells get increasingly smaller.
50.3K
01:31Internal Receptors
74.7K
Many cellular signals are hydrophilic and therefore cannot pass through the plasma membrane. However, small or hydrophobic signaling molecules can cross the hydrophobic core of the plasma membrane and bind to internal, or intracellular, receptors that reside within the cell. Many mammalian steroid hormones use this mechanism of cell signaling, as does nitric oxide (NO) gas.
74.7K
01:53RNA Stability
35.7K
Intact DNA strands can be found in fossils, while scientists sometimes struggle to keep RNA intact under laboratory conditions. The structural variations between RNA and DNA underlie the differences in their stability and longevity. Because DNA is double-stranded, it is inherently more stable. The single-stranded structure of RNA is less stable but also more flexible and can form weak internal bonds. Additionally, most RNAs in the cell are relatively short, while DNA can be up to 250 million...
35.7K
02:00Internal Energy
36.8K
The total of all possible kinds of energy present in a substance is called the internal energy (U), sometimes symbolized as E. Suppose a system with initial internal energy, Uinitial, undergoes a change in energy (transfer of work or heat), and the final internal energy of the system is Ufinal. Change in internal energy equals the difference between Ufinal and Uinitial.
36.8K