生体 細胞 の プログラム 可能な タンパク質 回路
Xiaojing J Gao1, Lucy S Chong1, Matthew S Kim1
1Howard Hughes Medical Institute, Division of Biology and Biological Engineering, Broad Center, California Institute of Technology, 1200 East California Boulevard, Pasadena, CA 91125, USA.
Science (New York, N.Y.)
|September 22, 2018
まとめ
合成生物学のモジュラーシステムを形成し,複雑な細胞機能を可能にします. このタンパク質回路エンジニアリングプラットフォームは ゲノム統合なしに 拡張可能なバイオテクノロジーを提供します
科学分野:
- 合成生物学
- 分子工学
- 哺乳類の細胞工学
背景:
- 複雑な合成回路が 必要になります
- 合理的な回路設計には不可欠です
- ウイルスのプロテアゼは,モジュラータンパク質の構成要素として潜在的可能性を秘めています.
研究 の 目的:
- 細胞機能を設計するための 合成可能なタンパク質-タンパク質 調節システムを開発する.
- モジュール式回路の構成要素として設計されたウイルスプロテアズの有用性を実証する.
- 哺乳類の細胞における タンパク質回路の設計のための 拡張性のあるプラットフォームを作成します
主な方法:
- エンジニアリングされたウイルスプロテアゼは,正方形のモジュールプロテアゼ (CHOMP) として機能するように設計されました.
- インプットプロテアゼは標的プロテアゼとドッキングして割れ,その機能を阻害するように設計された.
- CHOMPのコンポーネントは,規制カスケード,ロジックゲート,信号処理機能に組み込まれました.
主要な成果:
- CHOMPシステムは,哺乳類の細胞で様々な回路レベルの機能を成功裏に実装しました.
- 合理的に設計されたCHOMP回路は Ras腫瘍遺伝子の活性化に反応して細胞死を誘発した.
- CHOMP回路は単一のトランスクリプトとしてコード化され,ゲノム統合なしで提供できます.
結論:
- エンジニアリングされたウイルスプロテアゼは,合成タンパク質回路のための多機能で構成可能なプラットフォームを提供します.
- CHOMPシステムは,複雑な規制および信号処理機能の作成を可能にします.
- CHOMPはバイオテクノロジーにおける タンパク質回路工学のスケーラブルなアプローチを提供します
関連する概念動画
01:19Protein Dynamics in Living Cells
2.7K
Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...
2.7K
01:17Second-Order Circuits
3.6K
Integrating two fundamental energy storage elements in electrical circuits results in second-order circuits, encompassing RLC circuits and circuits with dual capacitors or inductors (RC and RL circuits). Second-order circuits are identified by second-order differential equations that link input and output signals.
Input signals typically originate from voltage or current sources, with the output often representing voltage across the capacitor and/or current through the inductor. For example, in...
Input signals typically originate from voltage or current sources, with the output often representing voltage across the capacitor and/or current through the inductor. For example, in...
3.6K
01:15First-Order Circuits
3.8K
First-order electrical circuits, which comprise resistors and a single energy storage element - either a capacitor or an inductor, are fundamental to many electronic systems. These circuits are governed by a first-order differential equation that describes the relationship between input and output signals.
One common example of a first-order circuit is the RC (resistor-capacitor) circuit. These circuits are used in relaxation oscillators such as neon lamp oscillator circuits. When voltage is...
One common example of a first-order circuit is the RC (resistor-capacitor) circuit. These circuits are used in relaxation oscillators such as neon lamp oscillator circuits. When voltage is...
3.8K
01:19The Y-to-Y Circuit
755
In a balanced four-wire wye-to-wye system, the arrangement involves wye-connected sinusoidal voltage sources and loads, connected through a neutral wire that links the neutral nodes of the source and load. The load impedance is connected across each phase of the load. The wye-connected source can be connected to the wye-connected load in four-wire and three-wire arrangements. A three-phase system is considered balanced when the load on each phase is equal, leading to uniform current flow and...
755
01:21LC Circuits
3.3K
An LC circuit consists of an inductor and a capacitor, either in series or parallel. Consider a charged capacitor connected with an inductor in series. Before the switch is closed, all the energy of the circuit is stored in the electric field of the capacitor. When the switch is closed, the capacitor begins to discharge, producing a current in the circuit. The current, in turn, creates a magnetic field in the inductor. Because of the induced emf in the inductor, the current cannot change...
3.3K
01:22Three-Phase Circuits
840
AC power distribution systems have three categories: single-phase, two-phase, and three-phase systems. The single-phase circuit, common in residential settings, typically employs a two-wire system connecting a single AC source to various loads. These circuits support standard household appliances operating at 120 volts (V) and 240 V, such as lamps, televisions, and microwaves. The first generators, Niagara Falls hydro plant installed in 1895, were two-phase and designed by Nikola Tesla. The...
840