人間の骨格幹細胞の識別
Charles K F Chan1, Gunsagar S Gulati1, Rahul Sinha2
1Department of Surgery, Stanford Medicine, Stanford, CA 94305, USA; Institute for Stem Cell Biology and Regenerative Medicine, Stanford Medicine, Stanford, CA 94305, USA.
Cell
|September 23, 2018
まとめ
研究者らは,自己再生する多能なヒト骨格幹細胞 (hSSCs) を骨,iPSC,脂肪組織から分離した. これらのhSSCは骨,軟骨,およびストロマを生成し,骨格の修復を助け,血液形成性幹細胞をサポートします.
科学分野:
- 再生医療
- 発達生物学
- 幹細胞生物学
背景:
- 人間の骨格幹細胞 (hSSC) の階層的な組織と調節はよく理解されていません.
- hSSCを特定し,特徴づけることは,骨格の発達と修復を理解するために不可欠です.
研究 の 目的:
- 自ら再生する多能なヒト骨格幹細胞 (hSSC) を分離し,特徴づけること.
- 様々な骨格系統を生成する hSSC の可能性と骨格損傷におけるその役割を調査する.
- ヒトとマウスの骨格幹細胞の遺伝子発現と表遺伝データを比較する.
主な方法:
- 胎児および成人ヒトの骨からhSSCを分離し,BMP2で治療された脂肪ストロマ (B-HAS) および誘発性多能幹細胞 (iPSC) を分離した.
- 単細胞の遺伝子発現分析
- 骨,軟骨,およびストロマに対するhSSCの分化の可能性の評価.
- 骨格損傷に対するhSSC反応に関するin vivo研究.
- 造血幹細胞維持のためのhSSC由来ストロマの分析.
- 人間とマウスの骨格幹細胞 (mSSCs) の遺伝子発現と表遺伝子の比較分析.
主要な成果:
- 骨,軟骨,ストロマを生成できるが,脂肪を生成しない自己再生性,多能性hSSCの分離
- hSSCは胎児と成人の骨で発見され,B-HASとiPSCから派生する可能性がある.
- 遺伝子発現分析は,胎児およびiPSC由来細胞における軟骨の分化に偏った,異なる源からのhSSC間の類似性を明らかにした.
- hSSCは,急性骨格損傷に対する反応として局所的な拡張を示した.
- hSSC由来ストロマサポートヒト造血幹細胞 (hHSC) の維持は,血清なしの条件で行われます.
- 人間とマウスのSSC間の骨格形成における保存されたと異なる経路の特定.
結論:
- 自ら再生する多能なヒト骨格幹細胞 (hSSC) の個別な集団が特定され,特徴づけられている.
- hSSCは骨格の修復に役割を果たし,血液形成幹細胞をサポートし,治療の可能性を強調します.
- 比較分析は,種間の骨格幹細胞機能の保存され,異なるメカニズムについての洞察を提供します.
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