生体細胞の超高解像度イメージングのための遠赤光活性化ボディパイ
Yang Zhang1, Ki-Hee Song, Sicheng Tang1
1Laboratory for Molecular Photonics, Department of Chemistry , University of Miami , 1301 Memorial Drive , Coral Gables , Florida 33146-0431 , United States.
Journal of the American Chemical Society
|September 25, 2018
まとめ
光活性化ボロンジピロメタン (BODIPY) フロロフォールは,生体細胞における単一分子イメージングを可能にします. これらの明るい赤色の探査機は,高解像度オルガネルの可視化のために,自己光と拡散の限界を克服します.
科学分野:
- 化学生物学
- バイオ物理学
- 光学イメージング
背景:
- ボロンジピロメタン (BODIPY) 染料はフルオフォールとして広く使用されている.
- 現存する光探査機は,しばしば光白化と限られた明るさに苦しんでいます.
- 生きている細胞で 高解像度画像を撮ることは 課題です
研究 の 目的:
- 生体細胞のイメージングのための新しい光活性化フッ素素を開発する.
- 単一分子検出と 細胞臓器の超高解像度イメージングを実現する.
- 従来の光顕微鏡の限界を克服するために
主な方法:
- 光活性化可能なオクサジン-BODIPY結合体の設計と合成
- 遠赤色光の光化学的活性化
- 生体細胞における単分子局所化顕微鏡 (SMLM)
- 細胞内にあるフッ素素の共性不動化
主要な成果:
- オクサジン部分の光誘発による解離は,BODIPYからの明るい遠赤色光を活性化します.
- フロロフォールは,リゾソームの区間に局所され,共動的に固定されます.
- 拡散抑制により,超高解像度の画像を繰り返し再構築できます.
- 高い空間解像度と位置精度でラベル付けされた臓器の可視化.
結論:
- 新しい光活性化ボディプイフローロフォアは 生体細胞で前例のない単一分子イメージングを可能にします
- 設計された探査機は,超解像度顕微鏡の拡散制限を克服します.
- このアプローチは,従来の光成像能力を超える空間解像度を大幅に高めます.
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