軸性ホックスコードはネマトステラベクトンシスの組織分割と体パターンを制御する
Shuonan He1, Florencia Del Viso1, Cheng-Yi Chen1
1Stowers Institute for Medical Research, Kansas City, MO 64110, USA.
まとめ
海藻のホックス遺伝子は 体の分割と触角の発達を制御します これは古代のホックス軸のコードが 動物の前後軸の進化に先立つことを示唆している.
科学分野:
- 発達生物学
- 進化生物学
- 遺伝学
背景:
- ホックス遺伝子は,前後 (A-P) 軸のパターン形成に不可欠である.
- ホックスコードの進化的起源は不明で,特にCnidariaのような決定的なA-P軸が欠けている基礎動物では不明である.
研究 の 目的:
- クニダリア,特に海藻ネマトステラベクトンシスの発達におけるホックス遺伝子の役割を調査する.
- 明確な A-P 軸がない場合に,ホックス遺伝子のネットワークが身体のパターニングとセグメンテーションにどのように貢献するのかを理解する.
主な方法:
- 短いヘアピンRNA (shRNA) 媒介のノックダウンを使用して,遺伝子発現を減少させました.
- CRISPR-Cas9 遺伝子編集を用いて 特定の遺伝子に変異を起こします
- ネマトステラベクトンシスの幼虫内皮の分割と触角パターンの遺伝子操作の効果を分析した.
主要な成果:
- Hox-Gbx遺伝子のネットワークが幼虫内皮の放射性分割を調節することを示した.
- Hox-Gbxの活動が失われると 指示軸に沿った触角のパターンに 欠陥が生じることが示されました
- 定義されたAP軸から独立して組織分割におけるホックス遺伝子の役割を特定した.
結論:
- 双極性A-P軸の進化の前に,軸性ホックスコードが身体のパターニングと組織分割に関与していたことを提案する.
- 分割におけるホックス遺伝子の基本的メカニズムが古くから保存されている可能性を示唆する.
- 発達遺伝子のネットワークの初期進化についての洞察を提供します.
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