関連する実験動画

Updated: May 6, 2026

In vivo and in vitro Studies of Adaptor-clathrin Interaction
17:14

In vivo and in vitro Studies of Adaptor-clathrin Interaction

Published on: January 27, 2011

13.8K

異なった配列決定因子 酵母真空プロテアゼの直接細胞内分類と改変

L M Johnson, V A Bankaitis, S D Emr

    Cell
    |March 13, 1987
    PubMed
    まとめ

    科学者たちは,カーボキシペプチダゼY (CPY) の特定の配列を特定し,その真空の分類を担当しました. この50アミノ酸の領域は,シグナルペプチドと真空分離シグナルを含み,CPYが正しい細胞目的地に到達することを保証します.

    さらに関連する動画

    Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae10:57

    Growth-based Determination and Biochemical Confirmation of Genetic Requirements for Protein Degradation in Saccharomyces cerevisiae

    Published on: February 16, 2015

    8.8K
    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae09:05

    Cycloheximide Chase Analysis of Protein Degradation in Saccharomyces cerevisiae

    Published on: April 18, 2016

    29.3K

    関連する概念動画

    Protein Complexes with Interchangeable Parts01:57

    Protein Complexes with Interchangeable Parts

    Groups of proteins may form a complex where each protein in this complex has a different role in the overall execution of the complex’s function. Often some of the proteins in the complex can be replaced by a closely related variant to give a complex that contains many of the same components yet is functionally distinct.
    The SCF ubiquitin ligase is a protein complex of five individual proteins. This complex attaches ubiquitin to other target proteins to mark them for degradation. In order...
    2.1K
    Intralumenal Vesicles and Multivesicular Bodies01:38

    Intralumenal Vesicles and Multivesicular Bodies

    Intraluminal vesicles (ILVs) are small vesicles 50-80 nm in diameter formed during the maturation of early endosomes. A specialized endosome containing numerous ILVs is called a multivesicular body (MVB). ILVs contain internalized molecules such as antigens, nucleic acids, proteins, and metabolites. Some of these molecules are released from the MVBs inside exosomes and are transported to other cells. Other MVBs contain molecules that are retained in the ILVs and are later degraded within the...
    4.0K
    Overview of Secretory Vesicles01:33

    Overview of Secretory Vesicles

    Secretory vesicles, also known as dense core vesicles (DCVs), are membrane-bound vesicles that transport secretory proteins, such as hormones or neurotransmitters. Regulated secretory vesicles transport proteins from the trans-Golgi network to the exterior of the cell. Proteins present in regulated secretory vesicles are required to be rapidly exocytosed in large amounts upon a specific stimulus.
    Various proteins regulate the aggregation of molecules inside the secretory vesicles. Chromogranins...
    8.9K
    Signal Sequences and Sorting Receptors01:41

    Signal Sequences and Sorting Receptors

    Signal sequences are short amino acid sequences that guide newly synthesized proteins to their proper location within the cell. Classical signal sequences are fifteen to sixty amino acids long and present at the N-terminus of a polypeptide chain. Each signal sequence has a conserved segment of basic residues towards their N terminus, a hydrophobic core, and a C-terminus rich in polar residues. The C-terminus also contains a signal cleavage site and features a -3 -1 sequence motif. The -3-1...
    9.9K
    Directing Proteins to the Rough Endoplasmic Reticulum01:34

    Directing Proteins to the Rough Endoplasmic Reticulum

    The organelle-specific signaling sequences direct proteins synthesized in the cytosol to their final destination like ER, mitochondria, peroxisomes, etc. Some of the proteins directed to ER are then trafficked via vesicles to other organelles within the cell or the extracellular environment through the Golgi complex. For example, the rough ER synthesizes soluble proteins for transportation to the lysosomes or secretion out of the cell. It can also synthesize transmembrane proteins that can...
    12.1K
    Export of Misfolded Proteins out of the ER01:32

    Export of Misfolded Proteins out of the ER

    After folding, the ER assesses the quality of secretory and membrane proteins. The correctly folded proteins are cleared by the calnexin cycle for transport to their final destination, while misfolded proteins are held back in the ER lumen. The ER chaperones attempt to unfold and refold the misfolded proteins but sometimes fail to achieve the correct native conformation. Such terminally misfolded proteins are then exported to the cytosol by ER-associated degradation or ERAD pathway for...
    4.3K