酵母HAP1活性化剤は,CYC1遺伝子の上流活性化部位UAS1に結合するために,因子RC2と競合する
Cell
|April 10, 1987
まとめ
酵母HAP1タンパク質は,CYC1遺伝子のアップストリーム活性化部位 (UAS1) に結合し,これはヘムによって刺激されるプロセスである. この研究では,HAP1,RC2,RAFをUAS1.1で相互作用する重要な要因として特定しています.
科学分野:
- 分子生物学は分子生物学である.
- イースト遺伝学 イースト遺伝学
- 遺伝子規制 遺伝子規制
背景:
- CYC1遺伝子は,細胞呼吸に不可欠な iso-1-cytochrome c をコードする.
- アップストリーム活性化部位1 (UAS1) は,CYC1遺伝子発現を制御する.
- HAP1タンパク質は,酵母における既知の転写活性化剤である.
研究 の 目的:
- 酵母HAP1タンパク質のCYC1遺伝子のUAS1へのインビトロ結合を調査する.
- UAS1と相互作用する他の要因を特定し,その役割を解明する.
- UAS1.1における規制要因の相互作用のモデルを提案する.
主な方法:
- 分割された酵母抽出物を用いたゲル電泳DNA結合測定法.
- 野生型および断片化されたHAP1を用いたインビトロ結合試験.
- UAS1の特定の領域 (Region B) に結合するHAP1の分析.
主要な成果:
- HAP1タンパク質はUAS1に直接in vitroで結合し,この結合はヘム刺激による.
- HAP1はUAS1のRegion Bと結合し,別の因子であるRC2の結合部位と重複する.
- HAP1とRC2の両方が,似たようなDNAの溝と相互作用する.
- UAS1のRegion Aに結合する新しい要因であるRegion A Factor (RAF) が特定されました.
結論:
- HAP1,RC2およびRAFは,UAS1.1経由でCYC1遺伝子を調節するために不可欠です.
- UAS1におけるこれらの要因の協力的および競争的相互作用のモデルが提案されています.
- これらの相互作用を理解することで,酵母遺伝子の調節に関する洞察が得られます.
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