関連する実験動画

Updated: May 6, 2026

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells
09:40

Production of Replication-Defective Retrovirus by Transient Transfection of 293T cells

Published on: December 4, 2007

19.0K

レトロウイルス感染によって処理された擬似遺伝子の作成

M Linial

    Cell
    |April 10, 1987
    PubMed
    まとめ

    この研究は,ルース・サルコマウイルスの変異体が,ネオ遺伝子mRNAのような細胞RNAをウイルスにパッケージ化できることを示しています. これは新しい細胞への遺伝子転送と統合を可能にし,新しいmRNA逆転写経路を示唆しています.

    さらに関連する動画

    Recombinant Retroviral Production and Infection of B Cells09:19

    Recombinant Retroviral Production and Infection of B Cells

    Published on: February 19, 2011

    13.8K
    Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites09:31

    Amplification, Next-generation Sequencing, and Genomic DNA Mapping of Retroviral Integration Sites

    Published on: March 22, 2016

    17.3K

    関連する概念動画

    Retrovirus Life Cycles01:10

    Retrovirus Life Cycles

    Retroviruses have a single-stranded RNA genome that undergoes a special form of replication. Once the retrovirus has entered the host cell, an enzyme called reverse transcriptase synthesizes double-stranded DNA from the retroviral RNA genome. This DNA copy of the genome is then integrated into the host’s genome inside the nucleus via an enzyme called integrase. Consequently, the retroviral genome is transcribed into RNA whenever the host’s genome is transcribed, allowing the...
    43.1K
    Retroviruses02:33

    Retroviruses

    Retroviruses and retrotransposons both insert copies of their genetic elements into the genome of the host cell. Thus, the viral genes are passed on when the host genome is replicated or translated. A typical retroviral DNA sequence contains 3-4 genes that encode the different proteins required for its structural assembly and function as a molecular parasite. This DNA is transcribed into a single mRNA, which is very similar in structure to conventional mRNAs, i.e., it is capped at the 5’...
    12.2K
    LTR Retrotransposons03:08

    LTR Retrotransposons

    LTR retrotransposons are class I transposable elements with long terminal repeats flanking an internal coding region. These elements are less abundant in mammals compared to other class I transposable elements. About 8 percent of human genomic DNA comprises LTR retrotransposons. Some of the common examples of LTR retrotransposons are Ty elements in yeast and Copia elements in Drosophila.
    The internal coding region of LTR retrotransposons and their mechanism of transposition closely resembles a...
    18.1K
    Mechanisms of Retrovirus-induced Cancers01:51

    Mechanisms of Retrovirus-induced Cancers

    Retroviruses are RNA viruses that have been shown to cause cancers in diverse species, including chickens, mice, cats, and monkeys. The RNA genomes of these viruses are first reverse-transcribed into single and then double-stranded DNA (dsDNA) copies. This dsDNA called proviral DNA then integrates into the host genome. Subsequently, the host cell transcribes the proviral DNA in concert with the chromosomal DNA. This leads to the production of viral RNA and proteins that assemble at the host...
    5.2K
    Rous Sarcoma Virus (RSV) and Cancer01:03

    Rous Sarcoma Virus (RSV) and Cancer

    Rous Sarcoma virus or RSV was discovered by F. Peyton Rous in the year 1911 as a filterable transmissible agent that could cause tumors in chickens. He won a Nobel Prize for this discovery in 1966. His experiments clearly demonstrated that some cancers could be caused by infectious agents and led to the discovery of many more cancer-causing viruses in animals as well as humans.
    RSV is a retrovirus that contains two copies of a plus-strand  RNA genome. Its genome consists of four main open...
    5.2K
    Size and Structure of Viral Genomes01:26

    Size and Structure of Viral Genomes

    Viral genomes exhibit remarkable diversity in size, structure, and composition, influencing their replication strategies and interactions with host cells. These genomes consist of either DNA or RNA and may be linear or circular. Additionally, they can be single-stranded or double-stranded, with each configuration affecting how the virus propagates within a host. RNA viruses, for instance, generally have smaller genomes than DNA viruses, a factor that contributes to their high mutation rates and...
    1.2K