移植後のマウスの単細胞レベルでの成像と再構築
Katie McDole1, Léo Guignard1, Fernando Amat1
1Janelia Research Campus, Howard Hughes Medical Institute, Ashburn, VA 20147, USA.
Cell
|October 16, 2018
まとめ
研究者はマウスの胚の発達中の細胞動態を追跡するために,先進的なライブイメージングと計算ツールを開発し,ガストルレーションとオルガノゲネシスを研究するための包括的なダイナミックアトラスを作成しました.
科学分野:
- 発達生物学
- 細胞ダイナミクス
- 画像技術
背景:
- ネズミの胚は哺乳類の発達を理解するために不可欠です.
- ガストルーレーションとオルガノゲネシス中の細胞行動の研究は,生画像と分析の限界のために困難です.
研究 の 目的:
- 開発中のマウスの胚の生体画像の 限界を克服するためです
- 細胞のダイナミクスを追跡する システムを作る
- 移植後のマウス発達のダイナミックなアトラスを構築する.
主な方法:
- 発達中のマウス胚を撮影するための自己適応光シート顕微鏡を開発した.
- 長期の細胞の軌跡と分裂を再構築するための コンピューティング・フレームワークを作成しました
- ダイナミックな運命マップと組織形態変異マップを生成した.
- 空間と時間における複数の胚の 細胞動態を記録し分析した.
主要な成果:
- 移植後のマウス胚の 細胞レベルでの発達を成功裏に捉えました
- 細胞の軌跡の復元と ダイナミックな運命の地図
- ガストルーレーションから初期オルガノゲネシスまでのマウス発達のダイナミックなアトラスを構築した.
- 資源として顕微鏡と計算方法を提供した.
結論:
- 開発された顕微鏡と計算ツールにより ネズミの胚の発達を 細胞レベルで前例のない方法で追跡できます
- ダイナミック・アトラスは 根本的な発達過程を研究するための貴重なリソースです
- この研究は,ガストルーレーションとオルガノゲネシス中の細胞の行動を調査する能力を向上させます.
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