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Transcription Factors02:16

Transcription Factors

82.7K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
82.7K
Eukaryotic Transcription Activators02:42

Eukaryotic Transcription Activators

12.8K
Transcription activators are proteins that promote the transcription of genes from DNA to RNA. In most cases, these proteins contain two separate domains ‒ a domain that binds to DNA and a domain for activating transcription; however, in some cases, a single domain is responsible for both binding and activation of transcription, as seen in the glucocorticoid receptor and MyoD.
The binding domains are capable of recognizing and interacting with regulatory sequences on the DNA. These...
12.8K
Transcription Elongation Factors02:35

Transcription Elongation Factors

13.9K
Transcription elongation is a dynamic process that alters depending upon the sequence heterogeneity of the DNA being transcribed. Hence, it is not surprising that the elongation complex's composition also varies along the way while transcribing a gene.
The transcription elongation is regulated via pausing of RNA polymerase on several occasions during transcription. In bacteria, these halts are necessary because the transcription of DNA into mRNA is coupled to the translation of that mRNA...
13.9K
Transcription Elongation Factors02:35

Transcription Elongation Factors

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4.8K
General Transcription Factors01:30

General Transcription Factors

7.1K
Tissue-specific transcription factors contribute to diverse cellular functions in mammals. For example, the gene for beta globin, a major component of hemoglobin, is present in all cells of the body. However, it is only expressed in red blood cells because the transcription factors that can bind to the promoter sequences of the beta globin gene are only expressed in these cells. Tissue-specific transcription factors also ensure that mutations in these factors may impair only the function of...
7.1K
Prokaryotic Transcriptional Activators and Repressors01:58

Prokaryotic Transcriptional Activators and Repressors

25.5K
The organization of prokaryotic genes in their genome is notably different from that of eukaryotes. Prokaryotic genes are organized, such that the genes for proteins involved in the same biochemical process or function are located together in groups. This group of genes, along with their regulatory elements, are collectively known as an operon. The functional genes in an operon are transcribed together to give a single strand of mRNA known as polycistronic mRNA.
Transcription of prokaryotic...
25.5K

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関連する実験動画

Updated: Feb 3, 2026

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation
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Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

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転写因子二分化によりp300アセチルトランスフェラーゼが活性化

Esther Ortega1, Srinivasan Rengachari1,2, Ziad Ibrahim1,3

  • 1European Molecular Biology Laboratory, Grenoble, France.

Nature
|October 17, 2018
PubMed
まとめ

転写因子二酸化は,トランス・オートアセチル化によってp300共同活性化剤を活性化させる. このプロセスは,ライシンに富んだ鍵のループを含み,遺伝子発現とクロマチンのアセチル化を調節する.

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters

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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome

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関連する実験動画

Last Updated: Feb 3, 2026

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12:54

Real-time Analysis of Transcription Factor Binding, Transcription, Translation, and Turnover to Display Global Events During Cellular Activation

Published on: March 7, 2018

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Rapid Synthesis and Screening of Chemically Activated Transcription Factors with GFP-based Reporters
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Describing a Transcription Factor Dependent Regulation of the MicroRNA Transcriptome
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科学分野:

  • 分子生物学
  • エピジェネティクス
  • タンパク質の生化学

背景:

  • p300はトランスクリプションの共同活性化剤であり,ヒストンアセチルトランスフェラーゼ (HAT) は遺伝子発現に不可欠です.
  • p300は,主に転写強化剤でクロマチンをアセチル化することによって機能する.
  • 信号経路によるp300活性化を制御する正確なメカニズムは,まだ完全に理解されていません.

研究 の 目的:

  • 転写因子リガンドがp300を活性化する分子メカニズムを解明する.
  • P300活性化における転写因子二分化の役割を調査する.
  • 結合体によるp300活性化の構造的基礎を特徴づける.

主な方法:

  • IRF3とSTAT1のモデルトランスクリプションファクタを使用した.
  • p300の活性化とアセチル化を研究するために生化学的測定を行った.
  • 活性化反応の中間物質を含むp300の結晶構造を決定した.

主要な成果:

  • 転写因子の二分化がp300の活性化に不可欠である.
  • ダイメリゼーションはp300のトランスオートアセチル化をそのオートインヒビトリーループ内で誘発する.
  • 結晶構造は,トランス・オートアセチル化の中間状態を明らかにし,RINGドメインの役割を強調する.

結論:

  • p300の活性化は,その転写因子リガンドの活性化と小分子化によって直接制御される.
  • この発見は,細胞シグナル伝達,転写因子活性,およびクロマチンのアセチル化との関連を説明する.
  • このメカニズムは,遺伝子の転写が表遺伝子レベルでどのように調節されているかについての洞察を提供します.