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関連する概念動画

Protein Kinases and Phosphatases02:54

Protein Kinases and Phosphatases

15.1K
Proteins undergo chemical modifications that trigger changes in the charge, structure, and conformation of the proteins. Phosphorylation, acetylation, glycosylation, nitrosylation, ubiquitination, lipidation, methylation, and proteolysis are various protein modifications that regulate protein activity. Such modifications are usually enzyme-driven.
Protein kinases
Many proteins in the cell are regulated by phosphorylation, the addition of a phosphate group. A family of enzymes called kinases...
15.1K
ATP Yield01:31

ATP Yield

78.9K
Cellular respiration produces 30 - 32 ATP per glucose molecule. Although most of the ATP results from oxidative phosphorylation and the electron transport chain (ETC), 4 ATP are gained beforehand (2 from glycolysis and 2 from the citric acid cycle).
The ETC is embedded in the inner mitochondrial membrane and is comprised of four main protein complexes and an ATP synthase. NADH and FADH2 pass electrons to these complexes, which pump protons into the intermembrane space. This distribution of...
78.9K
Hydrolysis of ATP01:08

Hydrolysis of ATP

81.4K
The bonds of adenosine triphosphate (ATP) can be broken through the addition of water, releasing one or two phosphate groups in an exergonic process called hydrolysis. This reaction liberates the energy in the bonds for use in the cell—for instance, to synthesize proteins from amino acids.
If one phosphate group is removed, a molecule of ADP—adenosine diphosphate—remains, along with inorganic phosphate. ADP can be further hydrolyzed to AMP—adenosine...
81.4K
Operational Amplifiers01:17

Operational Amplifiers

2.0K
The operational amplifier, often referred to as an op-amp, is a multifaceted building block of a circuit. This electronic component functions like a voltage-controlled voltage source and can also be used to create a voltage- or current-controlled current source. The design of an operational amplifier enables it to execute mathematical operations when external components like resistors and capacitors are linked to its terminals. An op-amp has the capacity to sum signals, amplify a signal,...
2.0K
MOSFET Amplifiers01:17

MOSFET Amplifiers

521
The MOSFET, when operating in its active region, functions as a voltage-controlled current source. In this region, the gate-to-source voltage controls the drain current. This principle underlies the operation of the transconductance MOSFET amplifier. The output current is directed through a load resistor to convert this amplifier into a voltage amplifier. The output voltage is then obtained by subtracting the voltage drop across the load resistance from the supply voltage. This process results...
521
BJT Amplifiers01:14

BJT Amplifiers

987
Bipolar Junction Transistors (BJTs) are pivotal components in amplifier circuits, functioning as voltage-controlled current sources in their active region. This characteristic allows them to efficiently control the collector current through variations in the base-emitter voltage. Essentially, BJTs amplify power due to their ability to take a weak input signal and output a much stronger signal.
In BJT amplifier configurations, particularly in common-emitter setups, the transistor's role...
987

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関連する実験動画

Updated: Feb 3, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.3K

ATPを反転させ,キナーゼ機能を強化する.

Joshua B Sheetz1, Mark A Lemmon1

  • 1Department of Pharmacology, Yale University School of Medicine, New Haven, CT 06520, USA; Cancer Biology Institute, Yale University, West Haven, CT 06516, USA.

Cell
|October 20, 2018
PubMed
まとめ

細胞シグナル伝達において重要な触媒残基を欠くタンパク質キナーゼは困難である. ある研究で,ある擬似キナーゼがアデノシン・モノフォスファート (AMP) を基板に転送し,キナーゼ折りの新しい触媒的機能を示していることが明らかになった.

科学分野:

  • 生物化学
  • 分子生物学
  • 細胞シグナリング

背景:

  • タンパク質キナーゼは 細胞のプロセスを調節する重要な酵素です
  • 偽キナーゼは,必須の触媒残留物がないタンパク質キナーゼのサブクラスである.
  • 複合的な細胞信号ネットワークを解読するには 擬似キナーゼの機能を理解することが不可欠です

研究 の 目的:

  • 特定のシドキナーゼの触媒活性と基板相互作用を調査する.
  • 仮キナーゼが正規のキナーゼ機能から逸脱するメカニズムを解明する.
  • 広範囲のキナーゼスーパーファミリー内の新しい触媒メカニズムを明らかにするために

主な方法:

  • 酵素活性を測定する生化学的測定法
  • タンパク質の結晶学で 構造の詳細を特定する
  • タンパク質の基質と改変を特定するための質量スペクトロメトリー

主要な成果:

  • 研究された偽キナーゼは,リン酸ではなくアデノシンモノフォスファート (AMP) をタンパク質基板に転送することが判明しました.
  • このAMP伝達活動は,キナーゼのような酵素の,以前に認識されていない触媒的機能を表しています.
  • 構造分析により,このユニークな触媒メカニズムに対応する 変化した活性部位の洞察が得られた.

さらに関連する動画

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

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Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.4K

関連する実験動画

Last Updated: Feb 3, 2026

Assaying Protein Kinase Activity with Radiolabeled ATP
08:05

Assaying Protein Kinase Activity with Radiolabeled ATP

Published on: May 26, 2017

19.3K
Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1
13:15

Experimental Approaches to Study Mitochondrial Localization and Function of a Nuclear Cell Cycle Kinase, Cdk1

Published on: February 25, 2016

12.4K
Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay
12:26

Identification of Cyclin-dependent Kinase 1 Specific Phosphorylation Sites by an In Vitro Kinase Assay

Published on: May 3, 2018

19.4K

結論:

  • 偽キナーゼは,これまで考えられていたより広範な触媒的レパートリーを示している.
  • キナーゼ折れは,正規のリン酸化を超えた様々な触媒的活動に対応できる.
  • この発見により 酵素の進化と 細胞の信号伝達の複雑さに関する 理解が広がりました