二機能免疫タンパク質は,FtsZを標的とするADPをリボシレートする毒素から細菌を保護する
See-Yeun Ting1, Dustin E Bosch2, Sarah M Mangiameli3
1Department of Microbiology, University of Washington School of Medicine, Seattle, WA 98195, USA.
Cell
|October 23, 2018
まとめ
バクテリアはADP-ribosylating毒素を使って 細胞分裂に不可欠なFtsZタンパク質を標的にします 発見された毒素はFtsZを改変し ポリメリゼーションを阻害し これらの細菌兵器に対する 新しい免疫メカニズムを明らかにします
科学分野:
- 微生物学
- 生物化学
- 構造生物学
背景:
- ADPリボシライゼーションは,タンパク質の機能に影響を与える重要な翻訳後の変化です.
- 細菌の毒素はADPリボシライゼーションを使用して,真核細胞のプロセスを破壊する.
- バクテリアの種間競争におけるADPリボシライゼーションの役割は,以前は未研究であった.
研究 の 目的:
- バクテリアの種間競争におけるADPリボシライゼーションの役割を調査する.
- バクテリアのADP-ribosylating毒素とその標的を特定し,特徴づけること.
- 毒素抑制と免疫のメカニズムを解明する.
主な方法:
- セラティア・プロテアマキュランスからのADPリボシレーティング毒素の特定
- 毒素阻害剤複合体の構造的決定
- FtsZポリメリゼーションと毒素活性を評価する生化学的測定法
- 毒素抑制と免疫メカニズムの分析
主要な成果:
- 種間の競争で使用される細菌のADP-ribosylating毒素を発見しました.
- セラティア・プロテアマキュランス毒素は,重要なFtsZタンパク質を改変し,ポリメリゼーションと細胞分裂を阻害することが示された.
- 活性部位閉塞と酵素によるADPリボース除去という2つの異なる抑制モードが明らかになった.
- ADP-リボースの除去によってもたらされる広範な免疫を非同類の毒素に対して示した.
結論:
- 細菌の軍拡競争は 新種の毒素防御システムの進化を促しました
- FtsZをADPリボシレーションで標的にすることは,細菌の競争のための戦略です.
- ADPリボースの酵素的除去は,細菌の細胞分裂機構を保護する広範囲の免疫を提供します.
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