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関連する概念動画

CRISPR01:59

CRISPR

57.9K
Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced...
57.9K
CRISPR and crRNAs02:53

CRISPR and crRNAs

19.1K
Bacteria and archaea are susceptible to viral infections just like eukaryotes; therefore, they have developed a unique adaptive immune system to protect themselves. Clustered regularly interspaced short palindromic repeats and CRISPR-associated proteins (CRISPR-Cas) are present in more than 45% of known bacteria and 90% of known archaea.
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...
19.1K
Single-pass Transmembrane Proteins01:25

Single-pass Transmembrane Proteins

6.6K
Integral membrane proteins are tightly associated with the cell membrane and play a crucial role in cell communication, signaling, adhesion, and transport of the molecules. Some integral membrane proteins are present only in the membrane monolayer. For example, the enzyme fatty acid amide hydrolase is present in the cytoplasmic side of the membrane monolayer. In contrast, another type of integral membrane protein, also known as a transmembrane protein, spans across the membrane. Transmembrane...
6.6K
Dimensional Analysis03:40

Dimensional Analysis

64.5K
Dimensional analysis, also known as the factor label method, is a versatile approach for mathematical operations. The main principle behind this approach is: the units of quantities must be subjected to the same mathematical operations as their associated numbers. This method can be applied to computations ranging from simple unit conversions to more complex and multi-step calculations involving several different quantities and their units.
Conversion Factors and Dimensional Analysis
The unit...
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Single-Strand DNA Binding Proteins01:03

Single-Strand DNA Binding Proteins

16.7K
For successful DNA replication, the unwinding of double-stranded DNA must be accompanied by stabilization and protection of the separated single strands of the DNA. This crucial task is performed by single-strand DNA-binding (SSB) proteins. They bind to the DNA in a sequence-independent manner, which means that the nitrogenous bases of the DNA need not be present in a specific order for binding of SSB proteins to it. The binding of SSB proteins straightens single-stranded DNA (ssDNA) and makes...
16.7K
Protein and Protein Structure02:15

Protein and Protein Structure

87.6K
Proteins are one of the most abundant organic molecules in living systems and have the most diverse range of functions of all macromolecules. Proteins may be structural, regulatory, contractile, or protective. They may serve in transport, storage, or membranes; or they may be toxins or enzymes. Their structures, like their functions, vary greatly. They are all, however, amino acid polymers arranged in a linear sequence.
A protein's shape is critical to its function. For example, an enzyme...
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関連する実験動画

Updated: Feb 3, 2026

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

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タンパク質のバーコードにより,高次元単細胞CRISPRスクリーンが可能になる

Aleksandra Wroblewska1, Maxime Dhainaut1, Benjamin Ben-Zvi2

  • 1Precision Immunology Institute, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA; Department of Genetics and Genomic Sciences, Icahn School of Medicine at Mount Sinai, New York, NY 10029, USA.

Cell
|October 23, 2018
PubMed
まとめ

研究者は,CRISPR遺伝子機能スクリーニングの限界を克服するために,新しいタンパク質バーコードシステム (Pro-Codes) を開発しました. この技術は高次元の単細胞フェノタイプ化が可能で 癌細胞の免疫編集と遺伝子調節に関する 新たな洞察を明らかにしています

キーワード:
CRISPRについてT細胞癌について機能的ゲノミクスインターフェロン・ガンマ経路マスサイトメトリープールスクリーンタンパク質のバーコード単細胞分析腫瘍免疫学

さらに関連する動画

Competitive Genomic Screens of Barcoded Yeast Libraries
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Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

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Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

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関連する実験動画

Last Updated: Feb 3, 2026

BEST: Barcode Enabled Sequencing of Tetrads
12:59

BEST: Barcode Enabled Sequencing of Tetrads

Published on: May 1, 2014

10.6K
Competitive Genomic Screens of Barcoded Yeast Libraries
11:59

Competitive Genomic Screens of Barcoded Yeast Libraries

Published on: August 11, 2011

18.8K
Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications
13:14

Genetic Barcoding with Fluorescent Proteins for Multiplexed Applications

Published on: April 14, 2015

9.8K

科学分野:

  • 分子生物学
  • 免疫学
  • バイオテクノロジー

背景:

  • CRISPRスクリーンは 遺伝子機能の発見に不可欠ですが DNAのバーコード解像度によって制限されています
  • 現在のDNAベースの方法は,フェノタイプ化能力を制限し,単細胞解像度が欠けている.

研究 の 目的:

  • 強化されたCRISPRスクリーニングのためのタンパク質レベルのバーコードシステム (Pro-Codes) を開発する.
  • より広範な機能的ゲノミクスアプリケーションのための高次元単細胞フェノタイプ化を可能にします.

主な方法:

  • 線形エピトープのトリプル組み合わせを用いた合成タンパク質バーコード (Pro-Codes)
  • 細胞にプロコードベクトルを導入し,14個の抗体でCyTOF質量サイトメトリを用いて分析した.
  • ノックアウト細胞におけるフォスフォシグナルを含むフェノタイプマーカーの同時分析のために,CRISPRとProcodeをペアリングする.

主要な成果:

  • >100個のユニークなプロコードを生成し,364個の集団を検出し,最大のタンパク質レポーターセットを作成しました.
  • Psmb8 と Rtp4 は,がん細胞の抗原依存免疫編集に不可欠であると特定されました.
  • プログラム死亡リガンド1 (Pd-l1) のネガティブレギュラーとしてSocs1を発見した.

結論:

  • Pro-Code技術により 単細胞解像度で何百もの遺伝子の 同時かつ高次元なフェノタイプ化が可能になります
  • この進歩により 機能的ゲノム学と薬剤発見の研究の 能力が大きく拡大しました