単一のサンプルでの多重免疫測定のための潜在的に解明された多色電化学発光
Weiliang Guo1, Hao Ding1, Chaoyue Gu2
1Institute of Analytical Chemistry, Department of Chemistry , Zhejiang University , Hangzhou 310058 , China.
Journal of the American Chemical Society
|November 2, 2018
まとめ
研究者は,電気化学発光 (ECL) マルチプレックス免疫測定のために新しいルテニウムとイリジウム複合体を開発した. この技術革新により,複数のバイオマーカーを同時に検出し,感度が向上し,サンプル量が減少することで臨床診断を進めることができます.
科学分野:
- 分析化学
- 材料科学
- 生物医学工学
背景:
- 電気化学発光 (ECL) は,臨床免疫測定において極めて重要であるが,ルミノフォアの可用性によって制限されている.
- マルチプレックス免疫測定では,同時にバイオマーカーを検出するには,多様な,区別可能な信号レポーターが必要です.
研究 の 目的:
- ECLの適用のための新しいルテニウム (II) とイリジウム (III) 複合体を合成し,特徴づけること.
- これらの新しいルミノフォールを利用したマルチプレックス免疫測定プラットフォームを開発する.
- マルチプレキシング機能のためのスペクトルとポテンシャル解像度ECL信号を調査する.
主な方法:
- 新しいRu (II) とIr (III) 複合体の合成と特徴付け
- 単体および混合光体ECLの行動 (強度,スペクトル,画像) の調査.
- ルミノフォールに負荷されたポリマービーズと均質なサンドイッチ免疫反応を用いたスポットフリーマルチプレックス免疫測定法の開発.
- パテンシャルとスペクトルの二重解像度ECLを使用して信号を読み取ります.
主要な成果:
- 異なるECL特性を有するいくつかの新しいRu (II) とIr (III) 複合体の合成が成功しました.
- 特定のRuとIr複合体間で最大145nmのスペクトルピーク分離を達成した.
- 混合ルミノフォアの潜在的解像度ECL信号を証明した.
- 300μLのサンプル容量で3つの抗原 (CEA,AFP,β-HCG) の同時検出をスポットフリーマルチプレックス免疫測定法で成功させた.
結論:
- 開発されたルミノフォールとマルチプレキシング戦略は,現在のECL免疫測定法の限界を克服しています.
- スポットフリーで二重解像度のECL免疫検査は,高通量バイオマーカーの検出に敏感で効率的なプラットフォームを提供します.
- この研究は,マルチルミノフォアECLの理解と,サンプル消費を減らす臨床診断におけるその応用を進める.
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