エンドサイトーシスをバイパスする: タンパク質の直接的な細胞分泌

Shubo Du^1,2, Si Si Liew¹, Lin Li³

¹Department of Chemistry , National University of Singapore , 117543 , Singapore.

Journal of the American Chemical Society

|November 3, 2018

まとめ

タンパク質治療は有望ですが細胞内での投与は困難です新しい方法は,細胞細胞質への治療性タンパク質の供給を改善するために,エンドソーマのトラッピングを回避することを目的としています.

科学分野:

バイオテクノロジー
分子生物学
薬物投与システム

背景:

タンパク質の治療用途はタンパク質工学の進歩により増加しています
タンパク質療法は高効能で特異性があり,腫瘍発生リスクは低い.
現在の制限は,プラズマ膜を通過できないため,治療用タンパク質を細胞外標的に制限しています.

研究の目的:

細胞内タンパク質を直接供給する戦略を検討する.
現在のタンパク質配送方法におけるエンドソーマルトラッピングの課題に対処する.
タンパク質の治療をバイパスする方法を研究する

主な方法:

細胞細胞質にタンパク質を直接輸送するための様々な戦略の議論.
エンドソーム・トラッピングを克服するための方法の分析
既存のおよび新しい細胞内投与技術の比較レビュー.

主要な成果:

細胞内タンパク質配送の現在のほとんどの戦略は, Endosomal Trapping の影響を受けています.
この内分体捕獲によりタンパク質の配分効率が非常に低い.
治療結果を改善するための潜在的な解決策です.

結論:

タンパク質治療の全力を発揮するには腸内細胞の捕獲を克服することが不可欠です
治療用タンパク質を細胞プラズマに直接輸送することは,内細胞経路をバイパスします.
これらの戦略のさらなる開発は,タンパク質ベースの治療法の進歩に不可欠です.

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