ヒトのIFN-αプロモーターから派生した配列によって強化剤の可逆的な静止作用
Cell
|September 25, 1987
まとめ
繰り返しrepAとrepBを含むIFN-alpha 1プロモーターのウイルス反応要素 (VRE) は,遺伝子発現を誘発することができる. 興味深いことに,特定のVRE配列は,誘導されていない条件下で転写を沈黙させます.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- ウイルス学 ウイルス学 ウイルス学
背景:
- インターフェロン-アルファ1 (IFN-アルファ1) プロモーターには,免疫遺伝子の活性化に不可欠なウイルス反応要素 (VRE) が含まれています.
- このVREは,不完全な19bpの2つの繰り返しで構成され,repAとrepBと指定されています.
研究 の 目的:
- 遺伝子誘導性におけるVRE (IFNα) とその構成要素の繰り返しの機能的役割を調査する.
- VRE配列が誘導転写と構成転写の両方に与える影響を調査する.
主な方法:
- 転写活性を評価するために,リポーター遺伝子アッセイを使用した.
- VRE (((IFN alpha),テトラメリックレパ,GAAAGT,AAGTGAを含む特定のDNA要素は,誘導性を授与する能力についてテストされました.
- SV40増強剤とSV40 72 bpリピートの転写への影響が調査されました.
主要な成果:
- テトラメリックレパA,GAAAGT,AAGTGAは,プロモーターの上流に配置されたとき,レポーター遺伝子に誘導性を授与しました.
- 誘発転写はSV40増強剤によって強化されたが,最小のプロモーターで弱かった.
- 驚くべきことに,これらの要素 (VREを除く) は,強化器とTATAボックスの間に置かれたとき,SV40強化器駆動の構成トランスクリプションを静止させ,誘導時に効果が廃止されました.
結論:
- VRE ((IFN alpha) とその次序は,ウイルス刺激への反応として遺伝子転写を調節する上で重要な役割を果たします.
- これらの要素は,特定の条件下で誘導性を授与し,また転写静止を媒介する能力を持つ二重の機能を備えています.
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