哺乳類のグルココルチコイド受容体誘導体は,酵母における転写を強化する
1Department of Biochemistry and Biophysics, University of California, San Francisco 94143.
まとめ
グルココルチコイド受容体誘導体は,哺乳類の細胞に類似して,酵母における遺伝子転写を強化する. これは,種間の受容体媒介の転写強化のための保存されたメカニズムを示唆しています.
科学分野:
- 分子生物学は分子生物学である.
- 遺伝学 遺伝学とは
- 細胞生物学 細胞生物学
背景:
- 哺乳類の細胞にあるグルココルチコイド受容体 (GR) は,グルココルチコイド応答要素 (GREs) と呼ばれる特定のDNA配列に結合する.
- GRがGREsと結合すると,関連遺伝子の転写が促進されます.
研究 の 目的:
- グルココルチコイド受容体誘導体が酵母における転写を強化できるかどうかを調査する.
- 酵母と哺乳類の細胞の間に受容体媒介の転写強化のメカニズムが保存されているかどうかを決定する.
主な方法:
- 酵母におけるグルココルチコイド受容体誘導体を発現する.
- GRE配列を酵母細胞クロームc1 (CYC1) プロモーターおよびその変異変異体と融合させる.
- 受容体によって媒介される転写強化の評価.
主要な成果:
- グルココルチコイド受容体誘導体は,酵母に発現すると,転写を成功裏に強化しました.
- トランスクリプションの強化は,上流アクティベーター配列が欠如した変異したCYC1プロモーターでも観察され,TATA領域のみを必要とする.
結論:
- グルココルチコイド受容体は,酵母と哺乳類の細胞の両方において共通で高度に保存されたメカニズムで作用する.
- この保存されたメカニズムは,真核細胞の転写制御の基本的側面を強調しています.
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