エンドプラズマ網膜に結合した膜のない臓器は,3'UTR媒介のタンパク質間相互作用を可能にします
1Cancer Biology and Genetics Program, Memorial Sloan Kettering Cancer Center, New York, NY 10065, USA.
Cell
|November 20, 2018
まとめ
タンパク質の多機能性を可能にする代替3' 未翻訳領域 (3' UTR) 研究者らは,TISの粒子を発見し,エンドプラズマ網膜はTIGERドメインを形成し,タンパク質の相互作用と多様性を高めています.
科学分野:
- 分子生物学
- 細胞生物学
- 生物化学
背景:
- ヒトの遺伝子には,代替の3' 未翻訳領域 (3' UTRs) が多く存在し,3' UTR媒介の相互作用によってタンパク質の多機能性を可能にします.
- 3'UTR情報がタンパク質の機能にどのように影響するかを理解することは,細胞の複雑性を解読するのに極めて重要です.
研究 の 目的:
- 3'UTRがタンパク質に情報を転送するメカニズムを調査する.
- タンパク質とタンパク質の相互作用と機能の調節に関与する新しい細胞区画を特定する.
主な方法:
- RNA結合タンパク質TIS11Bとその関連構造の特徴
- 顕微鏡検査と生化学検査で,TISの粒子の形成と,その内プラズマ網膜 (ER) との関連性を分析する.
- タンパク質とタンパク質の相互作用とタンパク質表面発現を媒介するTIGERドメインの役割を調査する.
主要な成果:
- RNA結合タンパク質TIS11Bは,AU豊富な元素を含むmRNAで濃縮された膜のない有機体であるTIS粒子を形成する.
- TISの粒子はERと結合し,TIGER領域と呼ばれる独特の亜細胞区画を形成する.
- TIGERドメインは,SETと膜タンパク質間の3'UTR媒介の相互作用を促進し,CD47やPD-L1のようなタンパク質の表面表現と機能的多様性を高める.
結論:
- TIGERドメインは,特定の,機能的に関連するタンパク質相互作用を促進する新しいサブセルラーコンパートメントを表します.
- このコンパートメントは,3' UTR駆動の相互作用を媒介することによって,タンパク質の表面表現と機能的多様性を高めます.
- 発見は,タンパク質の機能と細胞プロセスに影響を与える新しい転写後の調節層を明らかにしています.
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