細胞内酵素封じ込めのためのペプチドの指示された組立

Zhaoqianqi Feng¹, Huaimin Wang¹, Bing Xu¹

¹Department of Chemistry , Brandeis University , 415 South Street , Waltham , Massachusetts 02454 , United States.

Journal of the American Chemical Society

|November 20, 2018

まとめ

細胞内の酵素を隔離するためにペプチドアセンブリを用いて合成生物分子コンデンサットを作りましたこの新しいアプローチは細胞の自然な信号伝達を模倣し酵素相互作用の調節のための新しい戦略を提供します

科学分野:

生物化学
細胞生物学
超分子化学

背景:

細胞の信号伝達には液体のような生物マクロ分子が鍵となります
膜のないオルガネルの合成模倣をデザインすることは新興分野です
細胞内酵素封じ込めの現在の方法は限られている.

研究の目的:

ペプチドアセンブリを用いた生物分子凝縮物の合成模倣を開発する.
エンドプラズマ網膜 (ER) にある酵素の細胞内連鎖を調査する.
細胞内のタンパク質の相互作用を調節するための新しい戦略を探求する.

主な方法:

合成されたペプチドナプロクセン結合体が酵素前駆体として用いられる.
ホスファタゼによる酵素的脱酸化を用いて,水素ゲレータ形成と超分子組成を誘導した.
これらのアセンブリを使用してERの酵素コロカライゼーション (COX-2とPTP1B) を調査した.

主要な成果:

ERで細胞環境で超分子組成に成功しました
シクロオキシゲネーゼ-2 (COX-2) とタンパク質チロシンフォスファテーゼ1B (PTP1B) の細胞内連鎖が実証されている.
NSAID モチーフ,フォスフォチロジン,酵素脱リン化が酵素コロカライゼーションにとって重要であることが判明した.

結論:

小型のペプチドの超分子組成は,細胞内酵素連鎖のためのバイオ分子凝縮体を模倣することができます.
この研究は,細胞内の酵素を関連付ける上分子プロセスを使用する最初の例を示しています.
この発見は細胞内液体凝縮物と酵素相互作用の調節のための新しい戦略の洞察を提供します.

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