バイオエンジニアリングによる心臓血管バイパス移植の迅速な自己組み立て
Daniel von Bornstädt1, Hanjay Wang1, Michael J Paulsen1
1Departments of Cardiothoracic Surgery (D.v.B., H.W., M.J.P., A.B.G., A.E., A.T., L.S., A.N.S., V.N.T., K.J., C.H., Y.J.W.), Stanford University, CA.
Circulation
|November 27, 2018
まとめ
この研究は,生物分解性の組織接着剤のエスカフォードを使用して,自己組み立てられた工学血管管の生産を加速する新しい技術を導入します. この方法は生産時間を2週間まで大幅に短縮し,心臓血管バイパス移植の臨床適用を可能にします.
科学分野:
- バイオマテリアル科学
- 血管生物学
- 組織工学
背景:
- 心血管バイパス移植のための自律性動脈と静脈管は,患者の可用性によって制限されています.
- 自己組み立ての細胞のみの移植は 棚から入手可能ですが 長い製造時間を伴い 臨床使用を妨げています
- エンジニアリングされた血管管の生成を加速することは 臨床翻訳に不可欠です
研究 の 目的:
- 自己組織化された血管管の生産を大幅に加速するための新しい技術を導入する.
- 心血管バイパス移植におけるこれらの迅速な導管の有効性と安全性を評価する.
主な方法:
- 人間の大動脈の滑らかな筋肉の細胞と 繊維芽細胞を針の周りに包んで 管状の構造を作りました
- 臨床的に承認された生物分解性の組織接着膜は,バイオリアクターでの即時浸透と内皮化のための一時的な支架として使用されました.
- エンジニアリングされた血管管はネズミの股関節動脈インターポジション移植モデルで評価され,破裂圧,血管活性,流動力学,透かし性,および組織学が評価されました.
主要な成果:
- 粘着膜で安定した管は,安定していない対照と比較して5分の1で成熟した.
- 管は生理学的血管活性,500mmHg以上の破裂圧,および移植後8週間の100%の透かし性を示した.
- ヒストロジカル分析により, 異なる層と弾性膜を含む成熟した動脈構造が明らかになった.
結論:
- この新技術により 血管管の製造時間は2週間まで短縮され 心血管外科手術の臨床スケジュールと一致します
- この方法は臨床的に承認された材料を使用し,様々な細胞源に適応し,将来の臨床翻訳を容易にする.
- 迅速に生成される細胞ベースの血管移植は,動脈硬化症の治療に有望な可能性を示しています.
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