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関連する概念動画

C4 Pathway and CAM01:27

C4 Pathway and CAM

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Most plants use the C3 pathway for carbon fixation. However, some plants, such as sugar cane, corn, and cacti that grow in hot conditions, use alternative pathways to fix carbon and conserve energy loss due to photorespiration. Photorespiration is the process that occurs when the oxygen concentration is high. Under such conditions, the rubisco enzyme in the Calvin cycle binds O2 instead of CO2, which halts photosynthesis and consumes energy.
C4 Pathway
The C4 pathway is used by plants such as...
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Other Glycolytic Pathways01:24

Other Glycolytic Pathways

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The pentose phosphate pathway (PPP) operates in parallel with glycolysis, facilitating the metabolism of both pentoses and glucose. This pathway consists of two distinct phases: the oxidative and non-oxidative phases. While it does not directly generate ATP, the intermediates formed during the process can integrate into glycolysis, contributing to cellular energy metabolism when required.Oxidative Phase: NADPH ProductionThe oxidative phase of the pentose phosphate pathway is primarily...
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Auditory Pathway01:15

Auditory Pathway

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Auditory pathways constitute the complex neural circuits responsible for transmitting and interpreting auditory information from the peripheral auditory system to the brain. Sound waves are initially captured by the outer ear, funneled through the ear canal, and reach the tympanic membrane (eardrum). These vibrations are transmitted via the middle ear's ossicles to the inner ear's cochlea.
When viewed cross-sectionally, the cochlea reveals the scala vestibuli and scala tympani flanking...
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ER Retrieval Pathway01:45

ER Retrieval Pathway

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In the secretory pathway, vesicles transport proteins from one cellular compartment to another in forward transport to deliver the protein to its correct location. Occasionally, misfolded proteins and incorrect proteins escape their original compartments, and a retrieval pathway is used to return the escaped proteins to their original compartment.
The ER uses many checkpoints to prevent the entry of incorrectly folded or a resident protein as cargo onto a transport vesicle. These mechanisms...
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Respiration Pathways01:26

Respiration Pathways

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Cellular respiration is a fundamental metabolic process that enables organisms to generate energy from organic molecules. One of its central pathways is the tricarboxylic acid (TCA) cycle, also known as the Krebs cycle, which plays a crucial role in energy production and biosynthetic processes.Conversion of Pyruvate to Acetyl-CoAThe pyruvate generated from glycolysis undergoes oxidative decarboxylation by the pyruvate dehydrogenase complex, producing acetyl-CoA, one molecule of NADH, and one...
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Microtubule Associated Proteins (MAPs)01:42

Microtubule Associated Proteins (MAPs)

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Microtubule function and architecture are regulated by an array of specialized proteins called microtubule-associated proteins or MAPs. These proteins are widespread across different organisms and have conserved protein motifs, like the multi-TOG domain for tubulin binding found in the CLASP family of MAPs. Some MAPs are lineage-specific based on their conserved domains. Their functions depend upon the cytoskeletal architecture and cell type they are located within. In-plant cells, a specific...
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関連する実験動画

Updated: Feb 2, 2026

Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c
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Activation of Apoptosis by Cytoplasmic Microinjection of Cytochrome c

Published on: June 29, 2011

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サイトクロームP450における基質認識経路のマッピング

Navjeet Ahalawat1, Jagannath Mondal1

  • 1Tata Institute of Fundamental Research, Center for Interdisciplinary Sciences , Hyderabad 500107 , India.

Journal of the American Chemical Society
|November 28, 2018
PubMed
まとめ

サイトクロームP450酵素は,大きなタンパク質開口ではなく,基板誘発の構造変化を含む,単一の支配的な経路を利用します. これは,これらの重要な金属酵素の触媒センターに基板がどのように到達するかを明らかにします.

科学分野:

  • 生物化学
  • 酵素学
  • 構造生物学

背景:

  • サイトクロームP450は薬物の代謝と解毒に不可欠なメタロ酵素である.
  • 活性部位への基板アクセスのメカニズムは,特に閉じた結晶構造を考慮して,不明のままです.

研究 の 目的:

  • サイトクロームP450の構造的異質性により,基質へのアクセス経路が変化するかどうかを調べる.
  • シトクロームP450の触媒中心への基板の侵入のメカニズムを解明する.

主な方法:

  • 原子学的分子ダイナミクスシミュレーション (数マイクロ秒)
  • P450camとの自発的な基板関連性の分析
  • マルコフ状態モデリングと自由エネルギー分析

主要な成果:

  • P450camにおける基質の認識は,単一の,よく定義された支配的な経路によって行われる.
  • 基板結合には,キー表面チャネルと基板誘発のサイドチェーンが含まれる.
  • 触媒センターへのアクセスは,大規模なタンパク質開口を必要としません.

結論:

  • サイトクロームP450基板の認識は基板誘発の構造的再配置によって引き起こされる.

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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s
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A General Method for Detecting Nitrosamide Formation in the In Vitro Metabolism of Nitrosamines by Cytochrome P450s

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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells
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Measuring Mitochondrial Substrate Flux in Recombinant Perfringolysin O-Permeabilized Cells

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  • サイトクロームP450の基質へのアクセスに関する包括的なモデルが確立され,以前の発見を調和させています.